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High Throughput Screening Laboratory
Si RNA High Throughput Screening Proposal
Instructions: Please fill information in Section 1 and return to the KU-HTS contact Melinda Broward
(mbroward@kumc.edu). Information in Section 2 will be completed in meeting to be scheduled with
Principal Investigator and KU-HTS personnel.
Principal Investigator Information
Name: _______________________________________________________________________________
Institution: ____________________________________________________________________________
Department/Lab: _______________________________________________________________________
Screen Charge to information (charge code or bill to information):
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E-mail:_______________________________________________________________________________
Phone #:______________________________________________________________________________
Fax #:________________________________________________________________________________
Personnel working on project (name and position):_____________________________________________
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Biology, Rationale, and Purpose Screen
Provide a brief description of the biology, rationale, and goals for conducting the screen.
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Add any data that has been generated to support scientific validity of proposed assay (research design and
methods optimized, cell growth characteristics etc )
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Cite related publications/references
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Confidential
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Source of project funding (if any)
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Assay Protocol
Required number of experimental genes targeted for knock-down.
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Are there any safety concerns associated with cells? __________________________________________
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Si RNA delivery Assays:
Plate format: 384/ 96-well
Cell-line and cell origin
Number of cells per well at seeding
siRNA delivery reagent (Vendor/Catalog no.)
Attach files
siRNA delivery system (Reverse/Forward transfection)
siRNA complexing protocol
Number of cells per well at seeding
Time to assay point
Time of exposure to siRNA
Media changes during incubation (if any)
Cell harvesting process
Known efficiency of knockdown (qtPCR, rtPCR, other)
Cyxtoxicity measurements
Proposed plate map with controls
Description of controls
Reagents and medias
(Insert additional details of procedures)
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Screening Timetable and Logistics
Which libraries do you anticipate screening (Circle)?
Validation, Full genome, Cell Cycle, Kinase, Druggable Genome libraries
Is assay still being optimized?_____________________________________________________________
Timing issues, potential stop points_________________________________________________________
Confidential
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Stability and Process Studies______________________________________________________________
Reagent storage requirements and stability for projected duration of HTS work_______________________
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Reaction stability over projected assay time___________________________________________________
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Are reagents and supplies readily available, any necessary order lead times or production times?
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Proposed plate map and Incubation time (with compound):
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Provide a realistic estimate as to when assay will be ready for validation screening at HTS
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Technology Transfer and Intellectual Property (IP)
Have you talked to the Technology Transfer office about potential intellectual property associated with you
biological target or assay method? _________________________________________________________
Has a provisional patent been filed (date filed or contacted office)? ________________________________
Do you have an upcoming presentation or publication disclosing IP? ______________________________
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Data Handling
Do you need raw data only or data analyzed:________________________________________________
Do you want the data to be kept private or open to the KU HTS community (once IP protected and you have
published). Yes or No; If yes, then you will get to see other shared data and vice versa.
Confidential