Course
Course
Credit Hour
Lecture
Laboratory
Lecturers
: Fermentation Technology & Natural Product
: SY30303
:3
: 2 hours/week
: 3 hours/week
: Assoc. Prof. Dr. Jualang Azlan Gansau and Miss Roslina Jawan
Lecture Hall
Time
: BT51 (Bilik Tutorial 51, SST)
: Wednesday (10-12pm)
Synopsis
This course aims to complement the knowledge and concepts presented in SY20403 (Biochemical
Engineering). In this course the student is introduced to the more important topics about
fermentation process and apparatus design. It focuses on the downstream processing aspects of
biotechnology and will allow students to be aware of how products and processes are
commercialised. The central theme of this course is to provide students with knowledge and
understanding the technology, processes, regulatory requirements and commercial issues
involved in the production and commercialisation of biotechnology products. Topics to be
discussed include microbial growth techniques, isolation, presentation, media characteristics,
sterilization, large-scale cell culture, fermentation, downstream processing of products including
separation and purification technology, process design, standard operating procedures,
compliance and regulatory requirements and the commercialisation considerations in the design
of a downstream process are extensively taught. The course also will allow the students to
understand the relationship between other unit of operations and fermentation process.
Applications of fermentation technology were covered at the end of the syllabus. Natural
products topic also has been elaborated which include the classes and application of natural
products, isolation and purification, and determination of bioactivity of natural compounds.
Objectives







To introduce fermentation process to the students
To expose students to the application of fermentation technology in current industries
Study the sterilization techniques, microbial section, isolation and preservation
Understand the development of inoculum for industrial fermentations
Understand fermenter design
Understand the technologies use for recovery and purification of fermentation products
Know about effluent treatment
Assessment
Quiz
Practical/Tutorial Report
Assignment
Mid Semester Exam
Final Semester Exam
:
:
:
:
:
5%
15%
10%
30%
40%
Course Content
Week
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Scope of Lecture
An introduction to Fermentation Processes
Fermentation Pathways
The Isolation, Preservation and Improvement of Industrial microorganisms
Media for Industrial Fermentation; Sterilization; The Development of
Inoculum for Industrial Fermentations
Design of a Fermenter
Instrumentation and Control
Aeration and Agitation
MID SEMESTER BREAK
Effluent Treatment ; Fermentation Economics
Primary and Secondary Metabolism; Introduction to Natural Products
Chemistry
Classes and Functions of Secondary Products
Screening for Natural Product Activity
Modern Methods of Secondary Product Isolation and Analysis
Large-Scale Production of Secondary Metabolites in Plant Cell Cultures
Fermentation Applications (liquid and solid-waste management, microbial
mass production, primary metabolites production, secondary metabolites
production, etc.)
STUDY WEEK
FINAL EXAM
Remark
JAG
JAG
JAG
(Quiz)
JAG
RJ
RJ
(Quiz)
RJ
RJ
RJ
JAG
JAG
(Quiz)
JAG
JAG
JAG/RJ
(Assignment)
Practical Class
Practical
1
2
3
4
5
6
7
Practical Topic
Sauerkraut – A natural tradition fermentation
Substrate utilization rate of Saccharomyces cerevisae
Fermentation kinetic study of Saccharomyces cerevisae in batch culture
Fermentation study of Saccharomyces cerevisae in stirred-tank
fermenter
Natural products isolation, purification and biological activity test
Field-trip (Industrial visit)
Field trip (Industrial visit)
Remark
RJ
RJ
RJ
RJ
JAG
RJ
RJ
References
1. Baileys, J.E. and Ollis, D.F. 1986. Biochemical Engineering Fundamentals (2nd). McGraw-Hill
Inc. Singapore.
2. Doble, M. and Gummadi, S.N. 2007. Biochemical Engineering. Prentice-Hall of India Private
Limited. New Delhi.
3. Leland J. Cseke, Ara Kirakosyn, Peter B. Kaufman, Sara L. Warber, James A. Duke and Harry
L. Brielmann. 2006. Natural Product from Plants (2nd Edition). CRC Press, Taylor and Francis,
USA.
4. Lixin Zhang and Arnold L. Demain (Eds). 2005. Natural Products: Drug Discovery and
Therapeutic Medicine. Humana Press Inc, New Jersey, USA.
5. Rao, D.G. 2007. Introduction to Biochemical Engineering. Tata McGraw-Hill Publising Company
Limited. New Delhi.
6. Richard J. P. Cannel. 1998. Natural Product Isolation. Humana Press Inc, New Jersey, USA.
7. Stanbury, P. F. and Whitaker, A. 1984. Principles of Fermentation Technology. Pergamon
Press Limited. Oxford.