Microbial Fermentation - Quotation Inquiry Form Instructions Please answer as many questions as possible and email the completed form to protein@genscript.com. You can also fax it to 1-732-210-0262 or 1-732-885-5878. Assuming all required information has been provided, our service representative will get back to you with a free quote within 1 business day. Customer Information If you have registered an account with GenScript, please identify yourself by giving us your name and email address or the Account No. Name Account No. Phone Organization Shipping Address (necessary to determine shipping cost) Email Address Target name* Fermentation details* Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. ☐ Regular ☐ High cell density Fermentation volume Click here to enter text.L Need purified protein? ☐ Yes ☐ No If “Yes”, list Yield Click here to enter text.mg ☐ Sequence Click here to enter text. PurityClick here to enter text.% Enter DNA or protein sequence. We will perform codon optimization & gene synthesis. Please ensure sequence accuracy prior to submission. Due to formatting issues, occasionally, copypasting sequence directly into the sequence box may not be entirely accurate Starting material* ☐ DNA/Expression construct We will subclone if required and transform into the appropriate strain ☐ Glycerol stock Bacteria ☐ E. coli Fermentation organism* ☐ Bacillus sps Yeast ☐ S. cerevisiae ☐ Other Click here to enter text. ☐ P. pastoris ☐ Other Click here to enter text. If “Other”, please enter full name of bacteria or yeast species. Please note that we only accept non-sporulating, non-pathological strains for fermentation Characterization ☐ Endotoxin ☐ ELISA ☐ Other ☐ None ☐ HPLC ☐ IEF ☐ N-terminal sequencing SDS-PAGE and Western Blot will be used to determine fragment purity, A280 will be used to measure the concentration and MALDI-TOF peptide mapping will be used to determine protein identity (if required) Protein Intended Applications (select all that apply) ☐ Antigen Structural analysis ☐ Binding kinetics ☐ Display technologies ☐ Druggability studies ☐ Epitope Mapping ☐ Functionality studies ☐ Immunoassay ☐ Protein-receptor interaction studies ☐ Screening ☐ Other Click here to enter text. Approximate timeline for project initiation ☐ ASAP ☐ Within 1 month ☐ Within 3 months ☐ Within 6 months Click here to enter text. Comments Please enter any comments in this field *Fields required in order to process your quote quickly Terms and Conditions Standard deliverables include cell paste, batch records and QC datasheet. Synthetic gene in pUC57 will be delivered if gene synthesis is performed Gene synthesis and protein purification are add-on services that cost extra If gene synthesis is performed, codon optimization using our proprietary, OptimumGene™ technology, will be performed in order to increase recombinant protein expression We only accept non-sporulating, non-pathological strains for fermentation