Protein Expression and Purification Service Quotation Request Form
Instructions
Please answer all of the following questions and send the completed form to protein@genscript.com if you choose
bacterial, yeast, and/or baculovirus/insect cell expression system(s);
Send the completed form to bioprocess@genscript.com if you choose mammalian cell expression system.
Our service representative will contact you with a quote shortly.
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or
Account No.
Name:
Account No.:
Phone:
Organization:
Shipping address:
(Necessary to determine shipping cost)
Email address:
Protein Expression
Target protein:
Protein name:
Species:
Protein sequence: (crucial for evaluation)
MW:
or accession number:
Note: Please enter the protein sequence if mutants are included in your protein sequence.
Gene synthesis with codon optimization (For Human/Mouse/Rat ORF genes exist in NCBI
database, starting from $0.29/bp; for other genes, starting from $0.35/bp)
(Please enter your gene sequence:
)
Gene synthesis without codon optimization (For Human/Mouse/Rat ORF genes exist in
NCBI database, starting from $0.29/bp; for other genes, starting from $0.35/bp)
Starting material:
(Please enter your gene sequence:
)
Your supplied template DNA
(Please enter your gene sequence:
, vector name:
and attach vector map
(e.g. NTI file)
Your supplied expression ready construct
(Please enter your vector name:
, vector sequence:
, and attach vector map
(e.g. NTI file, please indicate your cloning site)
Others (Please specify):
Please indicate the preferred expression host system:
Bacteria
Yeast
Baculovirus/insect cell
Mammalian cell
If mammalian cell expression system is preferred, please select your preferred host cell
line:
HEK293
CHO
Others (Please specify):
No preference
Notes: If you DO NOT have preference, HEK293 or its derivatives will be used as default cell line in
our platform.
Expression system:
Has the gene been expressed in any host before?
Yes
No
If YES, please indicate the host:
Bacteria
Yeast
Baculovirus/insect cell
Mammalian cell
Others (Please specify):
Yield:
Solubility:
Protocol:
Other information:
Notes: The above information is crucial for us to evaluate your project.
Secretion signal
sequence: (For insect
cell and mammalian cell
expression systems)
Do you have any preferred secretion signal sequence for expressing your protein of
interest?
Its own signal peptide
No preference
Others (Please specify):
Please specify your preferred expression format:
Secretory expression only (GenScript will only purify the protein from the medium)
Protein purification from the cell lysate if very little protein can be obtained from the
medium (Extra fee is charged)
Expression evaluations on both secretory expression with the signal peptide and
intracellular expression without the signal peptide (Recommended)
Expression scale:
Protein properties:
Application(s) of your
protein: (Please select
all that apply)
Amount of protein:
mg or Volume of culture required:
liter(s)
Membrane-bound
Secreted
A protease
Other enzyme
Cytoplasmic protein
IgG antibody
Toxic to E.coli
Other features that may cause difficulty in either expression or purification (Please
specify):
High-throughput screening (HTS)
Activity assays
NMR
X-ray crystallography
Antigen
in vivo studies
Protein-protein interactions
Others (Please specify):
Comments:
Protein Purification
Product requirements:
Purity:
%
Endotoxin Limit:
Concentration:
Do you want GenScript to recommend a purification system for purifying your protein of
interest?
Yes
No, I want to choose my own purification system (Please specify):
Purification method:
Do you need GenScript to follow any specific expression and/or purification protocol?
Yes (Please attach your protocol:
)
No
Note: Your protocol will be very helpful to increase success rate and shorten the delivery time.
Do you want GenScript to select 1 or 2 tags (6xHis, GST, or MBP) to facilitate purification?
Yes
No, I want to choose my own tag(s) (Please specify):
Do you want GenScript to select additional 1 or 2 tags (Trx, TF, Dsb, or Nus) to increase
solubility and/or expression level? (Recommended—can greatly improve yield; the tag(s) will be
Tag(s) selection:
added to N-terminus)
Yes
No, I want to choose my own tag(s) (Please specify):
Do you want GenScript to add the tag(s) at the N-terminus?
Yes
No, I have designated place(s) to add the tag(s) (Please specify):
Note: If internal tag(s) is/are involved, please designate the position number(s) that corresponds to
the sequence of which the accession number you have indicated above.
Tag(s) removal:
Do you want GenScript to add a protease cleavage site between the tags and the target
protein?
Yes
No
If YES, please specify the protease you want to add:
GenScript’s choice
Enterokinase
PreScission
rTev
Thrombin
Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we DO NOT
recommend the introduction of protease cleavage site between the target protein and the C-terminal
tag.
Do you want GenScript to remove tag(s) in the purification step?
Yes
No
Small tag (e.g. His) can be retained after purification
Note: The recovery rate varies with the characteristics of the protein.
Refolding:
If the expressed protein is insoluble, do you want GenScript to attempt the refolding?
Yes
No
Note: GenScript will NOT charge extra fee for conventional refolding.
Protein
characterization:
Special requirements:
(information on buffers or
handling, etc.)
SDS-PAGE
IEF
N-terminal sequence
ELISA
MALDI-TOF
HPLC
Endotoxin
Others (Please specify):
GLP-compliant
service:
(for insect and mammalian
systems only!)
If Do you need GLP-compliant services?
Yes
No
Note: GLP-compliant services will result in additional fees!
Comments:
Project Information
Order initiation:
Pricing estimation
Immediately
Within six months
Quote for ordering
Within one month
For grant application purpose
Within three months