Protein expression
Project feasibility questionnaire
Instructions
Please fill in this form (one form per protein) and e-mail to proteomics.services@eurogentec.com and
please indicate in the subject line next to your original request “provided protein expression details”
Customer information
Name:
Institution:
Shipping address (for determination of shipping costs):
Phone:
Email:
Needed service
Expression host:
Purification:
Escherichia coli
Yes
Mammalian cells (HEK293T)
(CHO)
No
Please note that a Tag will be attached to the protein for purification.
The purified protein will be in the best buffer compatible with the solubility of the protein. If you need
a special buffer * or if a particular additive should be avoided, please inform us.
* This change will affect the protein solubility.
Do you want to receive the protein:
lyophilised
in solution
Protein information
Protein name:
Accession number:
Amino acid sequence (please provide exactly as to be purified, including the leader sequence):
Is your protein naturally secreted into the culture medium?
Yes
No (Please include an artificial leader in the sequence)
What is the predicted size of your protein in reduced form?
kDa
Will your protein migrate at the theoretical molecule size in SDS PAGE, or will it differ (e.g. due to
glycosylation?
Is your protein a monomer:
Expression scale:
Yes
No
mg purified protein needed
For protein production by transiently transfected HEK293 or CHO cells, a pilot service (small scale
purification) will be done first to evaluate the necessary purification work for the production of your
desired protein amount.
Gene Information
DNA sequence or Genbank accession number:
Is the gene needed to be codon-optimised for the appropriate system?
Comments
Yes
No