Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Protein Expression & Purification
Quotation Request Form
Page 1 of 4
Customer Information
If you have an existing account with ProteinCT, just fill in your name and email address or your Account No.
Name of Requestor:
Account No.:
Name of PI:
First:
Last:
Middle:
Title:
Organization:
Phone:
Fax:
Email Address:
Shipping Address
Project Information
Nature of Inquiry
Pricing estimation
Quote for ordering
Estimated Project
Initiation
Immediately
>6 months
1-3 months
3-6 months
Other, please specify
Research Use Only
Clinical Applications
Intended Use
For grant application purpose
Please note that we produce materials for research use only. We can perform developmental work for future
clinical applications, but are not GMP-certified.
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Protein Expression & Purification
Quotation Request Form
Page 2 of 4
Service Specifications
Please fill in project requirement details.
1.
General information
Target:
Protein name:
Accession number:
Species:
Protein sequence:
MW:
Please indicate mutations/variants as compared to the sequence corresponding to the accession number.
Target properties:
Membrane-bound
Secreted
Cytosolic
Protease
Other enzymes
IgG antibody
Other features to consider, please specify:
Nuclear
Toxic to E.coli
Pick an expression system:
Bacteria
Yeast
Baculovirus/insect cell
Mammalian cell
Transient Transfection
Stable cell line expression
Select a mammalian host cell line:
HEK293
CHO
Other (Please specify):
Periplasm
No preference
HEK293 or its derivatives will be used as default cell line if no preference is selected.
Expression system:
Has the protein been expressed previously?
Yes
No
If YES, please indicate the expression host:
Bacteria
Yeast
Baculovirus/insect cell
Mammalian cell
Other (Please specify):
Yield:
Solubility:
Protocol:
Other information:
Please provide your protocol and experience in a separate Word document. The information will be useful for
project evaluation and quotation.
Secretion signal
sequence:
For insect cell and
mammalian cell
expression systems
Expression scale:
Protein modification
Intended
application(s):
Select all that may apply
Comments:
Preferred secretion signal sequence:
Target’s own signal peptide
No preference
Other (Please specify):
Preferred expression/purification process:
Recommended: Parallel expression evaluation for both secretion with signal peptide and
intracellular expression without signal peptide
Secretion expression only (Purification of target protein from the expression medium only)
Purification from the cell lysate when very low purification yield from medium
Amount of protein:
mg or Volume of culture required:
liter(s)
Stable isotope labeling
15
13
N
C
Biotinylation
High-throughput screening (HTS)
As Antigen for antibody generation
Protein-protein interactions
Activity assay
NMR
in vivo studies
Other (Please specify):
X-ray crystallography
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Protein Expression & Purification
Quotation Request Form
Page 3 of 4
Service Specifications
Please fill in project requirement details.
2.
Expression Construct Generation
Recommended: Synthesize and construct the expression vector by ProteinCT:
Gene synthesis with codon optimization (recommended)
Organism for codon optimization:
Enter target gene sequence:
Gene synthesis without codon optimization
Enter target gene sequence:
Starting material:
Subclone from User supplied template DNA - Vector name:
Please attach vector map (e.g. NTI file)
Enter your gene sequence:
User supplied expression ready construct - Expression vector name:
Please attach vector map (e.g. NTI file), indicating your desired cloning site
Enter vector sequence:
Other (Please specify):
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Protein Expression & Purification
Quotation Request Form
Page 4 of 4
Service Specifications
Please fill in project requirement details.
3.
Protein Purification and Characterization
Protein purity:
%
QC requirements: Protein Concentration:
Endotoxin Level:
Buffer:
1xPBS (default)
mg/ml
Other, please specify
ProteinCT will develop the purification method/system for you (default option):
Yes
No, use my own purification method/system; Please specify:
Purification method:
Follow user supplied purification protocol:
Yes (Please attach your protocol in a separate Word document:
Fusion tag:
)
ProteinCT will use the best combination of N-terminal and/or C-terminal tags to enhance
expression (default option):
Yes
No, use user specified tag(s); Please specify:
Add a protease cleavage site between the tags and the target protein:
Yes
No
If YES, please specify desired protease:
ProteinCT choice (default)
Enterokinase
PreScission
rTev
Tag removal:
No
Thrombin
Please note that extra amino acids may be left on target protein
Fusion tag removal:
Yes
No
Leave small tags (e.g. His) on target protein
Please note that protein yield may be reduced after tag removal
Refolding:
Protein characterization:
Special requirements:
e.g. buffer composition,
shipping/packaging/handling
When target protein is expressed insoluble, refolding will be performed to increase yield:
Yes
No
SDS-PAGE
Native PAGE
Silver staining
Western blot
ELISA
HPLC
Endotoxin measurement
N-terminal sequencing
MALDI-TOF
Others (Please specify):