Protein Expression & Purification
Custom Service Requirements Form
Please submit completed form to geneartprotein@lifetech.com.
* Mandatory fields for Quote generation
Contact Information*:
Name:
Organization:
Address:
Email:
Phone Number:
General Information:
Expression only:
Deliver Frozen Cell Pellet
Deliver Conditioned Medium
Service(s)desired*:
Expression and Purification
Target*:
Protein Name:
Accession #:
or
Other:
Protein Sequence:
Note: Sequence information is mandatory for Quote generation
Target Properties:
Species:
Biosaftey Level:
MW:
Molecular extinction coefficient (ε):
Expected Localization:
Cytosolic
Other:
Secreted
unknown
Nuclear
Transmembrane
Please note: Purification Services are restricted to non-membrane bound proteins
Expression System
desired*:
Secretion Leader:
please add artificial leader
Freestyle™CHO
Freestyle™293
Baculovirus, Sf9 (Bac-to-Bac®)
no preference
Expi™293
no preference, please suggest
Comments:
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SECTION I
Expression plasmid construction
Start with Gene Synthesis (recommended to optimize expression)
Expression plasmid already stored at Thermo Fisher
Starting Material:
Expression plasmid provided by client
Other:
Note: In case the starting material will be provided by client, please provide full sequence of expression
construct.
Location of Tag:
N-term
C-term
Affinity Tag(s):
no preference, please suggest
Preferred Tag to be used
6xHis
GST
Strep
Other:
no preference, please suggest
Tag cleavage required?
Yes:
No
Comments:
SECTION II
Expression
1L Pilot Study (standard starting volume)
Expression Scale*:
2L
3L
Other:
4L
5L
10L
20L
50L
Comments:
SECTION III Purification
Desired purity:
Product
Requirements:
Note: No guarantees for the above requirements can be given before pilot study has been
completed
Preparative SEC
Storage Buffer Composition:
Final Formulation:
Concentration:
Endotoxin Limit:
Analytical SEC (HPLC)
Additional
Purification Steps
Yield needed:
Other:
none
Other:
no preference, please apply standard (PBS)
Dispensing Size:
no preference, please apply standard (2mL aliquots)
Storage Temp.:
no preference, please apply standard (dry ice)
Comments:
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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