Republic of the Philippines
DEPARTMENT OF SCIENCE AND TECHNOLOGY
PHILIPPINE SCIENCE HIGH SCHOOL
ILOCOS REGION CAMPUS
CHEMISTRY 4: FRONTIERS IN CHEMISTRY
Dry Laboratory Activity: Introduction to High Performance Liquid Chromatography (HPLC)
Instructions: Answer each questions given below substantially WITHOUT going over 200 words.
Guide Questions:
1. Name 5 parts/components of a high performance liquid chromatography (HPLC) setup and give their
functions. (5pts)
2. Explain the concept that governs the HPLC method. (5pts)
3. Give advantages of HPLC over Paper Chromatography. (5pts)
4. What are different types of HPLC detectors, and suggest possible detectors applicable to
phytochemicals. Support your answer. (5pts)
5. A chromatogram of an isomeric alcohol mixture composing of n-butanol, 2-methyl-1-propanol, 2-butanol,
and tert-butyl alcohol is presented below. The solvent system used was chloroform-ethanol in a 4:1
proportion. Draw the structure of each alcohol and identify which of the peaks correspond to the
component of the alcohol mixture. Explain your basis on your peak assignments. (25pts)
a
b
c
d