Application for use of the Small Molecule Screening Facility
Revised by NRP 2010
User Information:
Name
E-mail
Principal Investigator Email:
Telephone #
Lab Name (Name of PI)
Cancer Center Member? Yes
No
( circle one)
Billing Information:
Billing Contact Name:
Billing Contact Phone:
Billing Contact Email:
Grant to charge:
The SMSF will obtain funding information from your billing contact and supply a copy
of each invoice. By applying for SMSF services, I understand that all activities
performed at the SMSF incur charges as outlined below, and a fund account
number will be provided within 1 week of receiving an invoice. I understand that if a
fund account number is not provided within 1 week, that additional billable hours may be
generated to cover administrative time required to obtain a fund account number.
Signature of Principal Investigator: ________________________________________
Note: A copy will be sent to your billing contact for their reference.
SMSF Rates and Policies:
All users of the SMSF and the MCCF pay user fees to offset the costs of staffing,
instrument maintenance, and operational expenses. Initial project consulting is
provided free of charge (not to exceed 2 hours). User participation is encouraged at
all times.
Hourly Staff Time for Assisted Facility and Equipment Use, Chemistry and
Screening Consulting, reporting and data analysis:
UW Investigator:
Off Campus Investigator:
$150.00 per hour* **
$300.00 per hour**
Hourly Staff Time for User Assisted Facility and Equipment Use (UW Member
Only)***:
UW Investigator
$75 per hour* **
Application for use of the Small Molecule Screening Facility
Revised by NRP 2010
*Members of the Carbone Cancer Center receive 25% subsidy of all project costs
including consumables and reagent costs.
**Users pay for all consumables and reagents required for their project.
***Large liquid handlers, HPLC, chemical libraries, and RNAi libraries are restricted
and must be handled/operated by facility staff
Walk-up:
Access to facility instruments without the assistance of staff is available for some
instruments. Charges are applied for amount of time reserved. A complete listing of
instruments is available on our website: http://www.hts.wisc.edu/scheduling.php
Compound Access Fees:
To offset the costs associated with maintaining the compound libraries, a small fee is
charged per library:
KBA01-08: $200
Chembridge: $500
Maybridge: $500
Chemdiv: $600
Life Chemicals $700
Life Chemicals 2: $700
Charges are based upon the size of the library. Charge is assessed 1X per project.
RNAi Access Fees:
To offset the costs associated with maintaining the RNAi libraries, a $2500 is charged for
use of the libraries.
Cytotoxicity and Cell Proliferation Assay Costs per Compound:
UW Investigator:
$175 per compound for preliminary 2 dose test in a cell panel including 2 of the
following cell lines: Du145, MCF7, HT-29, Hep3B, A549, SF-268, MB-MDA-231.*
An additional $175 per compound for full IC50 in 1-7 cell lines listed above.
An additional $350 will be charged for the controls per plate of 9 compounds ($175
preliminary assay/$175 full screen). *
To be sure we cover the costs to maintain the cells, there is a 9 compound/1 control
minimum, if less compounds are tested the minimum cost is $1750.*
For additional cell lines, $50 per compound per cell line, again 9 cpd/1 control minimum
or $500.
Application for use of the Small Molecule Screening Facility
Revised by NRP 2010
*Carbone Cancer Center members receive a 25% subsidy of all project costs including
consumables and reagent costs.
Non-UW Investigators:
$400 per compound for preliminary 2 dose test in a cell panel including: Du145, MCF7,
HT-29, Hep3B, A549, SF-268, MB-MDA-231.
An additional $400 per compound for full IC50 in 1- 7 cell lines listed above.
An additional $600 will be charged for the control per plate of 9 compounds tested in
preliminary and full IC50 screens.
To be sure we cover the costs to maintain the cells, there is a 9 compound/1 control
minimum, if less compounds are tested the minimum cost is $4000.00.*
For additional cell lines, $125 per additional cpd/cell line, 9 cpd/1 control minimum or
$1000.
Service Information:
1. Briefly describe your intended use of the SMSF (HTS, cytotoxicity assay, etc.).
2. For HTS, RNAi, and Mechanism assays please summarize your project:
3. Please briefly describe your protocol in general terms. (Eg 1. Add 30 L labeled proteins in buffer to
384 well plates. 2. Add 1 L 1mM compound to each well. 3. Incubate at room temp. 1hr. 4. Read in plate reader using LANCE
protocol.) (N/A if for Walk-up Instruments)
4. Please list materials needed at the screening facility for your project (plates, filter
wavelenths, etc.) –note all materials costs will be billed to the investigator.
Application for use of the Small Molecule Screening Facility
Revised by NRP 2010