Molecular Biology Services
PCR Product Cloning Submission Form
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II
Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
* Required fields
CUSTOMER DETAILS
*Name:
Reference ID:
Previous PCR Optimization Reference
ID (if any)
*Institute Name / Department
*Address:
*Contact Number:
*Email Address:
(Office)
(Mobile)
Principal Investigator / Supervisor:
Please fill in where applicable.
^SAMPLE INFORMATION
S/N
PCR Product Name
Size
(bp)
Purity
(State P for Purified
or U for Unpurified)
Concentration
(ng/µl)
(If purified sample
given)
OD ratio 260/280
(If purified sample
given)
Total volume
(µl)
1.
2.
3.
4.
5.
^ If you have a large number of samples, please attach a separate sheet with the details as requested in the above table.
** Name of Vector:
(1) pJET1.2: ampicillin: blunt; (2) pBASE-1: ampicillin: blunt (3) If other vectors are required, please request for Sub-Cloning Service (MBS-3002)
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Name of Vector**
(please specify either
pJET1.2 or pBASE-1)
Molecular Biology Services
PCR Product Cloning Submission Form
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II
Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
ADDITIONAL SERVICES (Additional fees are applicable)
Quantity
Stab Culture
Glycerol Stock
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
Services
Maxiprep, minimum yield is 100 µg
Note:
1. 1st BASE will perform quality checks on samples upon receipt.
2. If cloning fails, a set up fee for the PCR step will still apply.
3. Final plasmid construct will be delivered as 2 x 5 µg as lyophilized DNA. Service will also include sequencing data and Service Report.
Have I met the sample preparation requirements?
Checklist (Please indicate with a X):
Submitted ≥ 100 ng of purified PCR product (DNA) or ≥ 50 µl of unpurified PCR reaction in either TE or 10mM Tris-HCl (pH8.0) Elution Buffer
Purified DNA meets OD260/280= 1.8 to 2.0
Attached Gel Photo - 2 µl of PCR products should show a bright band. Please indicate DNA ladder, amount/volume of PCR product on gel, target band.
Samples submitted in 1.5ml microcentrifuge tubes with at least 10 µl of contents. Each tube should be clearly labeled using a permanent marker, with caps
sealed with parafilm. DNA should be shipped chilled.
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