Molecular Biology Services
Primer Walking Order Form
1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
*Required Fields
CUSTOMER DETAILS
*Name:
Reference ID:
*Institute Name / Department
*Address:
*Contact Number:
(Office)
(Mobile)
*Email Address:
Principal Investigator / Supervisor:
TYPE OF SERVICE (please indicate with an “X”)
Service Name
Primer Walking of Constructs – Single Pass
Remarks
- Plasmid Preparation
- Primer Synthesis
- Sequencing, 1 to 1.5 kb/ week
Primer Walking of Constructs – Bi-Directional
- Plasmid Preparation
- Primer Synthesis
- Sequencing, 1 to 1.5 kb/ week
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1st BASE Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs-sg@base-asia.com
Molecular Biology Services
Primer Walking Order Form
First BASE Laboratories Sdn Bhd (604944-X)
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
SAMPLE
S/N
Name
Type*
PRIMER WALKING
Total Size
(bp)
#
Antibiotic
Read Size
Sequence of Initial Sequencing Primers (5’ to 3’)
(bp)
1
Forward
Reverse
2
Forward
Reverse
3
Forward
Reverse
* Type of DNA: 1 = Purified Plasmid; 2 = Purified Fosmid/Cosmid; 3 = Purified BAC
# Ampicillin, Kanamycin, Chloramphenicol provided as free.
ADDITIONAL SERVICES (A separate fee is chargeable)
Quantity
Services
DNA verification by Restriction Digestion
Glycerol Stock
Have I met the sample preparation requirements?
Checklist (Please indicate with an “X”)
Submit ≥ 5 g (100 ng/l, 50 l) of purified plasmid in either TE or 10 mM Tris-HCl (pH8.0) Elution Buffer
Samples submitted in 1.5 ml microcentrifuge tubes. Each tube should be clearly labeled using a permanent marker, with caps
sealed with parafilm. Purified plasmid can be shipped at room temperature.
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Difficult
Region? (Y /
N / Unknown)