Molecular Biology Services Primer Walking Order Form Axil Scientific Pte Ltd 41 Science Park Road, #01-22/23 The Gemini Singapore Science Park II, Singapore 117610 T: +65 6775 7318 F: +65 6775 7211 E: mbs@axilscientific.com First BASE Laboratories Sdn Bhd (604944-X) Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana, Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia T: +603 8943 3252 F: +603 8943 3243 E: mbs-my@base-asia.com *Required Fields CUSTOMER DETAILS *Name: Reference ID: *Institute Name / Department *Address: *Contact Number: (Office) (Mobile) *Email Address: Principal Investigator / Supervisor: TYPE OF SERVICE (please indicate with an “X”) Service Name Primer Walking of Constructs – Single Pass Remarks - Plasmid Preparation - Primer Synthesis - Sequencing, 1 to 1.5 kb/ week Primer Walking of Constructs – Bi-Directional - Plasmid Preparation - Primer Synthesis - Sequencing, 1 to 1.5 kb/ week Page 1 of 3 Axil Scientific Pte Ltd 41 Science Park Road, #01-22/23 The Gemini Singapore Science Park II, Singapore 117610 T: +65 6775 7318 F: +65 6775 7211 E: mbs@axilscientific.com Molecular Biology Services Primer Walking Order Form First BASE Laboratories Sdn Bhd (604944-X) Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana, Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia T: +603 8943 3252 F: +603 8943 3243 E: mbs-my@base-asia.com SAMPLE S/N Name Type* PRIMER WALKING Total Size (bp) Antibiotic# Read Size Sequence of Initial Sequencing Primers (5’ to 3’) (bp) 1 Forward Reverse 2 Forward Reverse 3 Forward Reverse * Type of DNA: 1 = Purified Plasmid; 2 = Purified Fosmid/Cosmid; 3 = Purified BAC # Ampicillin, Kanamycin, Chloramphenicol provided as free. ADDITIONAL SERVICES (A separate fee is chargeable) Quantity Services DNA verification by Restriction Digestion Glycerol Stock Have I met the sample preparation requirements? Checklist (Please indicate with an “X”) Submit ≥ 5 g (100 ng/l, 50 l) of purified plasmid in either TE or 10 mM Tris-HCl (pH8.0) Elution Buffer Samples submitted in 1.5 ml microcentrifuge tubes. Each tube should be clearly labeled using a permanent marker, with caps sealed with parafilm. Purified plasmid can be shipped at room temperature. Page 2 of 3 Difficult Region? (Y / N / Unknown) Molecular Biology Services Primer Walking Order Form Axil Scientific Pte Ltd 41 Science Park Road, #01-22/23 The Gemini Singapore Science Park II, Singapore 117610 T: +65 6775 7318 F: +65 6775 7211 E: mbs@axilscientific.com Page 3 of 3 First BASE Laboratories Sdn Bhd (604944-X) Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana, Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia T: +603 8943 3252 F: +603 8943 3243 E: mbs-my@base-asia.com