Sequencing Mitochondrial Control Region PCR Products
1.
In a single 0.2 mL microcentrifuge tube combine:
Genome Lab Master Mix
Mito Forward Primer
Purified PCR reaction
Sterile, dI water
8 L
2.5 L
200 ng (or up to 9.5 L)
to 20 L Total Volume
2.
Place in thermal cycler with same cycling parameters as for the amplification of
the control region.
3.
The instructor will perform the following:
Ethanol precipitate the samples, and resuspend in 40 L of Sample Loading
Solution.
Load the samples onto a Beckman DNA Analyzer.