Sequencing Mitochondrial Control Region PCR Products 1. In a single 0.2 mL microcentrifuge tube combine: Genome Lab Master Mix Mito Forward Primer Purified PCR reaction Sterile, dI water 8 L 2.5 L 200 ng (or up to 9.5 L) to 20 L Total Volume 2. Place in thermal cycler with same cycling parameters as for the amplification of the control region. 3. The instructor will perform the following: Ethanol precipitate the samples, and resuspend in 40 L of Sample Loading Solution. Load the samples onto a Beckman DNA Analyzer.