Molecular Biology Services
PCR Product Cloning Submission Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
First BASE Laboratories Sdn Bhd
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
* Required fields
CUSTOMER DETAILS
*Name:
Reference ID:
Previous PCR Optimization Reference ID (if any)
*Institute Name / Department
*Address:
*Contact Number:
(Office)
(Mobile)
*Email Address:
Principal Investigator / Supervisor:
PCR Product Cloning Services
Quantity
Services
MBS-3002 PCR Product Cloning Service (up to 1.5kb) *1 positive clone sent for plasmid sequencing
MBS-3003 PCR Product Cloning Service (3kb to 5kb) *1 positive clone sent for plasmid sequencing
MBS-3004 Amplify and Purify PCR Products Prior to Cloning Services *Customer to provide PCR protocol and DNA template.
MBS-3005 Additional screening for positive clone. *24 colonies picked
MBS-3006 PCR Product Cloning Service PLUS (up to 1.5kb) *5 positive clones sent for colony PCR product sequencing
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Molecular Biology Services
PCR Product Cloning Submission Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
First BASE Laboratories Sdn Bhd
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
Please fill in where applicable.
SAMPLE INFORMATION
S/N
PCR Product Name
Size
Types
Total volume
Name of Vector**
(unpurified only)
(bp)
(please indicate with an “X”)
(µl)
(please tick)
3’ A-overhang
Blunt-end
1.
2.
3.
4.
5.
^ If you have a large number of samples, please attach a separate sheet with the details as requested in the above table.
** Name of Vector: (1) pJET1.2: ampicillin: blunt; (2) pBASE-1: ampicillin (3) Other vector, please enquire.
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pBASE-1
pJET1.2
Molecular Biology Services
PCR Product Cloning Submission Form
Axil Scientific Pte Ltd
41 Science Park Road, #01-22/23 The Gemini
Singapore Science Park II, Singapore 117610
T: +65 6775 7318
F: +65 6775 7211
E: mbs@axilscientific.com
First BASE Laboratories Sdn Bhd
Lot 7-1 to 7-3, Jalan SP 2/7, Taman Serdang Perdana,
Seksyen 2, 43300 Seri Kembangan, Selangor, Malaysia
T: +603 8943 3252
F: +603 8943 3243
E: mbs-my@base-asia.com
ADDITIONAL SERVICES (Additional fees are applicable)
Quantity
Services
MBS-4004 Stab Culture
MBS-4005 Glycerol Stock
MBS-4003 Maxiprep, minimum yield is 100 µg
Note:
1. 1st BASE will perform quality checks on samples upon receipt.
2. Final plasmid construct will be delivered as 2 x 5 µg as lyophilized DNA. Service will also include sequencing data and Service Report.
Have I met the sample preparation requirements?
Checklist (Please indicate with a X):
Submitted ≥ 100 l of unpurified PCR product (DNA)
Attached Gel Photo - 2 µl of PCR products should show a bright band. Please indicate DNA ladder, amount/volume of PCR product on gel, target band.
Samples submitted in 1.5ml microcentrifuge tubes with at least 10 µl of contents. Each tube should be clearly labeled using a permanent marker, with caps
sealed with parafilm. DNA should be shipped chilled.
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