Supplement Table A1 (see below).
Campostoma pauciradii and Nocomis biguttatus displayed significantly lower -amylase Km
values than N. asper and N. micropogon; none of the other taxa were different from one another.
Nocomis asper displayed a higher trypsin Km than any of the 10 other taxa. Campostoma
ornatum had a significantly lower trypsin Km than C. anomalum and N. leptocephalus-C, and
none of the remaining taxa had trypsin Km values that differed from each other (Supplement
Table S1). Individuals of C. ornatum, C. oligolepis, C. pauciradii, and N. micropogon had lipase
Km values that were not different from another, and all were significantly greater than the lipase
Km of C. anomalum, N. biguttatus, N. asper, and N. leptocephalus-H. Of these taxa, only the
lipase Km of N. asper was significantly different (greater) than that of N. leptocephalus-H
(Supplement Table S1).
Table A1. Michaelis-Menten constants (Km) for three digestive enzymes in species of the minnow genera Campostoma and Nocomis.
-Amylase
Species
Trypsin
Km
N
C. anomalum
0.22 ± 0.03ab
10
C. ornatum
0.20 ± 0.02ab
C. oligolepis
Km
Lipase
N
Km
N
0.38 ± 0.05b
10
0.48 ± 0.13ab
10
10
0.07 ± 0.02a
7
1.04 ± 0.21c
10
0.22 ± 0.04ab
10
0.10 ± 0.01ab
10
0.90 ± 0.07c
10
C. pauciradii
0.18 ± 0.03a
11
0.11 ± 0.02ab
11
1.00 ± 0.17c
11
N. biguttatus
0.17 ± 0.03a
4
0.20 ± 0.03ab
4
0.38 ± 0.09ab
4
N. asper
0.38 ± 0.07b
7
1.49 ± 0.75c
5
0.48 ± 0.13b
7
N. micropogon
0.43 ± 0.08b
6
0.27 ± 0.10ab
6
1.02 ± 0.19c
6
N. platyrhynchus
0.34 ± 0.22ab
4
0.25 ± 0.11ab
4
0.66 ± 0.12bc
4
N. leptocephalus (c)
0.27 ± 0.05ab
12
0.39 ± 0.11b
11
0.67 ± 0.13bc
12
N. leptocephalus (h)
0.26 ± 0.05ab
10
0.23 ± 0.04ab
10
0.23 ± 0.03a
9
F9, 83 = 2.09
--
F9, 79 = 4.96
--
F9, 82 = 5.20
--
P = 0.04
--
P < 0.01
--
P < 0.01
--
Values are mean (± SEM). Interspecific comparisons were analyzed with one-way ANOVA and Tukey’s HSD with a family error
rate of P=0.05.