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1. Accession number: 20084311651805
Title: Development of an immunoaffinity column method using broad-specificity monoclonal antibodies
for simultaneous extraction and cleanup of quinolone and sulfonamide antibiotics in animal muscle tissues
Authors: Li, Cun1, 2, 3 ; Wang, Zhanhui1, 2 ; Cao, Xingyuan1, 2 ; Beier, Ross C.4 ; Zhang, Suxia1, 2 ;
Ding, Shuangyang1, 2 ; Li, Xiaowei1, 2 ; Shen, Jianzhong1, 2
Author affiliation: 1 Department of Pharmacology and Toxicology, College of Veterinary Medicine,
China Agricultural University, Beijing, 100094, China
2 National Reference Laboratories for Veterinary Drug Residue, District Haidian, Beijing 100094, China
3 Department of Veterinary Medicine, College of Agriculture, Hebei University of Engineering, Handan,
056038, China
4 Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of
Agriculture, 2881 F and B Road, College Station, TX 77845-4988, United States
Corresponding author: Shen, J. (sjz@cau.edu.cn)
Source title: Journal of Chromatography A
Abbreviated source title: J. Chromatogr. A
Volume: 1209
Issue: 1-2
Issue date: October 31, 2008
Publication year: 2008
Pages: 1-9
Language: English
ISSN: 00219673
CODEN: JCRAEY
Document type: Journal article (JA)
Publisher: Elsevier, P.O. Box 211, Amsterdam, 1000 AE, Netherlands
Abstract: This paper describes a novel mixed-bed immunoaffinity column (IAC) method. The IAC was
produced by coupling anti-quinolone and anti-sulfonamide broad-specificity monoclonal antibodies to
Sepharose 4B for simultaneously isolating 13 quinolones (QNs) and 6 sulfonamides (SAs) from swine and
chicken muscle tissues, followed by antibiotic determination using liquid chromatography-tandem mass
spectrometry (LC-MS/MS). A new broad-specificity Mab (B1A4E8) toward sulfonamides was produced
using sulfamethoxazole as hapten that demonstrated cross-reactivities to 6 SAs in the range of 31-112%.
IAC optimized conditions were found that allowed the IAC to be reused for selective binding of both SAs
and QNs. Recoveries of all 19 antibiotics from animal muscle ranged from 72.6 to 107.6%, with RSDs
below 11.3% and 15.4% for intra-day and inter-day experiments, respectively. The limit of quantification
ranged from 0.5 to 3.0 ng/g. The strategy used here for a mixed-bed IAC may be used to study other
compounds and more than two classes of analytes simultaneously. © 2008 Elsevier B.V.
Number of references: 37
Main heading: Sulfur compounds
Controlled terms: Amides - Antibiotics - Antibodies - Chromatographic analysis - Columns
(structural) - Gel permeation chromatography - High performance liquid chromatography Histology - Liquid chromatography - Mass spectrometry - Monoclonal antibodies - Muscle
- Surface plasmon resonance
Uncontrolled terms: Immunoaffinity - LC-ESI-MS/MS - Muscle tissues - Quinolones 中国科技信息研究所
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Residue analysis - Sulfonamides
Classification code: 741.1 Light/Optics - 801 Chemistry - 802.3 Chemical Operations - 804.1
Organic Compounds - 804.2 Inorganic Compounds - 931.3 Atomic and Molecular Physics 943.3 Special Purpose Instruments - 711 Electromagnetic Waves - 408.2 Structural Members and
Shapes - 461.2 Biological Materials and Tissue Engineering - 461.3 Biomechanics, Bionics and
Biomimetics - 461.6 Medicine and Pharmacology - 461.8 Biotechnology - 461.9 Biology 461.9.1 Immunology
DOI: 10.1016/j.chroma.2008.08.116
Database: Compendex
Compilation and indexing terms, © 2009 Elsevier Inc.
2. Accession number: 20090511881850
Title: Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two
monoclonal antibodies
Authors: Li, Bingyu1, 2 ; Li, Cun1 ; Jiang, Haiyang1 ; Wang, Zhahnui1 ; Cao, Xingyuan1 ; Zhao, Sijun1 ;
Zhang, Suxia1 ; Shen, Jianzhong1
Author affiliation: 1 China Agricultural University, Department of Pharmacology and Toxicology,
College of Veterinary Medicine, Beijing 100094, China
2 Hebei University of Engineering, Department of Veterinary Medicine, College of Agriculture, Handan
056038, China
Corresponding author: Shen, J. (sjz@cau.edu.cn)
Source title: Journal of AOAC International
Abbreviated source title: J AOAC Int
Volume: 91
Issue: 6
Issue date: November/December 2008
Publication year: 2008
Pages: 1488-1493
Language: English
ISSN: 10603271
CODEN: JAINEE
Document type: Journal article (JA)
Publisher: AOAC International, 481 North Frederick Avenue - Suite 500, Gaithersburg, MD 20877-2417,
United States
Abstract: An immunoaffinity chromatographic method was developed for the purification of 9
sulfonamides (sulfamethazine, sulfadimethoxine, sulfamerazine, sulfathiazole, sulfamethoxazole,
suifamethizole, sulfadiazine, sulfamonomethoxine, and sulfapyridine) from chicken tissue (muscle and liver)
samples. Two monoclonal antibodies (antisulfamethazine and antisulfamethoxazole) were simultaneously
covalently coupled to CNBr-activated Sepharose 4B for the preparation of a re-usable immunoaffinity
column. After extraction with methanol-water and purification by immunoaffinity column, the sulfonamides
were determined by reversed-phase liquid chromatography and UV detection at 270 nm. The recoveries for
each drug at fortification levels of 10-50 ng/g ranged from 74.1 to 108.9% with relative standard deviations
from 1.9 to 11.5%. The limits of detection were 2 ng/g for each drug.
中国科技信息研究所
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Number of references: 25
Main heading: Antibodies
Controlled terms:
Amides
Chromatographic analysis
Chromatography
Column
chromatography - Drug products - Extraction - Gel permeation chromatography - Liquid
chromatography - Methanol - Military engineering
- Monoclonal antibodies - Muscle Purification - Solvent extraction - Sulfur compounds - Surface plasmon resonance
Uncontrolled terms: Immunoaffinity chromatography - Immunoaffinity column (IAC) - Sepharose
4B - UV detection
Classification code: 931.3 Atomic and Molecular Physics - 804.2 Inorganic Compounds - 804.1
Organic Compounds - 802.3 Chemical Operations - 801 Chemistry - 741.1 Light/Optics - 711
Electromagnetic Waves - 461.9.1 Immunology - 461.8 Biotechnology - 461.6 Medicine and
Pharmacology - 461.3 Biomechanics, Bionics and Biomimetics - 461.2 Biological Materials and
Tissue Engineering - 404.1 Military Engineering
Database: Compendex
Compilation and indexing terms, © 2009 Elsevier Inc.
3. Accession number: 20095312589110
Title: Rapid simultaneous determination of dexamethasone and betamethasone in milk by liquid
chromatography tandem mass spectrometry with isotope dilution
Authors: Li, Cun1 ; Wu, Yinliang2 ; Yang, Ting2 ; Zhang, Yan2
Author affiliation: 1 College of Agriculture, Hebei University of Engineering, Handan, 056038, China
2 Ningbo Agriculture Products Quality Detector Center, Ningbo, 315040, China
Corresponding author: Wu, Y. (wupaddyfield@tom.com)
Source title: Journal of Chromatography A
Abbreviated source title: J. Chromatogr. A
Volume: 1217
Issue: 3
Issue date: January 15, 2010
Publication year: 2010
Pages: 411-414
Language: English
ISSN: 00219673
CODEN: JCRAEY
Document type: Journal article (JA)
Publisher: Elsevier, P.O. Box 211, Amsterdam, 1000 AE, Netherlands
Abstract: A simple, sensitive and reliable analytical method for the rapid simultaneous determination of
dexamethasone and betamethasone in milk by high performance liquid chromatography-negative
electrospray ionization tandem mass spectrometry (HPLC-NESI-MS/MS) with isotope dilution was
developed. Samples were directly purified through C18 cartridge. Then the eluate was dried under nitrogen
and residues were dissolved in mobile phase. Samples were analyzed by HPLC-MS/MS on a Hypercarb
graphite column with a mixture of acetonitrile-water-formic acid as mobile phase. The samples were
quantified using dexamethasone-D4 as an internal standard. The procedure was validated according to the
European Union regulation 2002/657/EC determining specificity, decision limit (CCα), detection capability
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(CCβ), trueness, precision, linearity and stability. The method is demonstrated to be suitable for the
determination of dexamethasone and betamethasone in milk. The total time required for the analysis of one
sample was about 35 min. © 2009 Elsevier B.V. All rights reserved.
Number of references: 27
Main heading: High performance liquid chromatography
Controlled terms: Acetonitrile - Chromatographic analysis - Chromatography - Dilution Dissolution - Electrospray ionization - Graphite - High pressure liquid chromatography Isotopes - Liquids - Mass spectrometers - Mass spectrometry - Organic acids
Uncontrolled terms: 2002/657/EC - Analytical method - Betamethasone - Decision limit Detection capability - Dexamethasones - Electrospray ionization tandem mass spectrometry European Union regulations - HPLC-MS/MS - Hypercarb
- Internal standards - Isotope
dilutions - Liquid chromatography-tandem mass spectrometry - Mobile phase - Simultaneous
determinations
Classification code: 943.3 Special Purpose Instruments - 931.2 Physical Properties of Gases, Liquids
and Solids - 813.2 Coating Materials - 812.2 Refractories - 804.1 Organic Compounds - 804
Chemical Products Generally - 803 Chemical Agents and Basic Industrial Chemicals - 802.3
Chemical Operations - 802.2 Chemical Reactions - 801 Chemistry - 482.2 Minerals
DOI: 10.1016/j.chroma.2009.12.015
Database: Compendex
Compilation and indexing terms, © 2009 Elsevier Inc.
© 2009 Elsevier Inc. All rights reserved.
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