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Type of SOP: ☐ Process ☒ Hazardous Chemical ☐ Hazardous Class
Purpose
2,4-dinitrophenylhydrazine is a Potentially Explosive Chemical (PEC). It is used in the Brady’s test to qualitatively check for carbonyl groups associated with aldehydes and ketones. A positive test for an aldehyde or ketone functional group will yield a yellow, orange, or red precipitate. For aromatic carbonyl compounds, the precipitate will be red; for aliphatic carbonyl compounds, the precipitate will be yellow.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 119-26-6
Class: Potentially explosive chemical, flammable solid
Molecular Formula: C
6
H
6
N
4
O
4
Form (physical state): Powder
Color: Red
Boiling point: N/A
Potential Hazards/Toxicity
Target Organs: Lungs, nervous system, mucous membranes and blood
2,4-Dinitrophenylhydrazine
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Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

Potential Health Effects:
Eye: May cause eye irritation
Skin: Harmful if absorbed through skin. May cause skin irritation
Ingestion: Harmful if swallowed
Inhalation: May be harmful if inhaled. May cause respiratory tract irritation.
Chronic: Repeated or prolonged exposure to the substance can produce target organs damage
Engineering Controls
Use 2,4-dinitrophenylhydrazine in a fume hood to keep exposure to airborne contaminants below the exposure limit.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. ( http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/ )
Hand Protection
Butyl gloves are preferred, but nitrile gloves are also acceptable.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with 2,4-dinitrophenylhydrazine
Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR http://www.allsafetyproducts.biz/page/74172
OR http://www.showabestglove.com/site/default.aspx
OR http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses.
Skin and Body Protection
Flame resistant/cotton lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed..
Hygiene Measures
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before reuse.
First Aid Procedures
2,4-Dinitrophenylhydrazine
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Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

If inhaled
Evacuate the victim to a safe area as soon as possible. Loosen tight clothing such as a collar, tie, belt or waistband. If breathing is difficult, administer oxygen. If the victim is not breathing, perform mouth-to-mouth resuscitation. Seek medical attention.
In case of skin contact
After contact with skin, wash immediately with plenty of water for at least 15 minutes. Gently and thoroughly wash the contaminated skin with running water and non-abrasive soap. Be particularly careful to clean folds, crevices, creases and groin. Cover the irritated skin with an emollient. If irritation persists, seek medical attention. Wash contaminated clothing before reusing.
In case of eye contact
Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
If swallowed
Call the poison control center at 1-800-222-1222. Do not induce vomiting. Examine the lips and mouth to ascertain whether the tissues are damaged, a possible indication that the toxic material was ingested; the absence of such signs, however, is not conclusive. Loosen tight clothing such as a collar, tie, belt or waistband.
If the victim is not breathing, perform mouth-to-mouth resuscitation. Seek immediate medical attention.
Special Handling and Storage Requirements
Precautions for safe handling
Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed. Keep away from sources of ignition – No smoking. Take measures to prevent the buildup of electrostatic charge.
Conditions for safe storage
Flammable materials should be stored in a separate safety storage cabinet or room. Keep away from heat.
Keep away from sources of ignition. Keep container tightly closed. Keep in a cool, well-ventilated place.
Ground all equipment containing material. Keep container dry. Keep in a cool place.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-
8522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-
8522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
2,4-Dinitrophenylhydrazine
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Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-
222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-
1300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885-
7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
2,4-Dinitrophenylhydrazine
4
Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
2,4-Dinitrophenylhydrazine
5
Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.