UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
4-Nitrobiphenyl
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☐ Process
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☒Hazardous Chemical
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Purpose
4-Nitrobiphenyl is a mutagen and possible carcinogen. It was once used an in intermediate for dyes, a
plasticizer for cellulosics, as well as a wood preservative. It is no longer used or manufactured in the United
States.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 92-93-3
Class: Mutagen, Carcinogen
Molecular Formula: C12H9NO2
Form (physical state): Solid crystals
Color: White to yellow
Boiling point: 340 ºC
Potential Hazards/Toxicity
It is a known mutagen, experimental carcinogen in laboratory animals and probable human carcinogen.
4-Nitrobiphenyl
1
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Engineering Controls
A functioning certified ducted fume hood with adequate ventilation is required for all procedures involving 4Nitrobiphenyl.
Personal Protective Equipment (PPE)
Respiratory Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Nitrile gloves or an appropriate alternative are recommended when working with 4-nitrobiphenyl.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with 4-Nitrobiphenyl
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or goggles.
Skin and Body Protection
Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be
buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure
while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that
are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before
reuse.
First Aid Procedures
If inhaled
May cause respiratory tract irritation. The toxicological properties of this substance have not been fully
investigated.
In case of skin contact
Remove contaminated materials (gloves, clothing, etc.) and wash the affected area with water for at least 15
minutes.
In case of eye contact
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower
eyelids. Get medical aid
4-Nitrobiphenyl
2
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
If swallowed
May cause irritation of the digestive tract. The toxicological properties of this substance have not been fully
investigated.
Special Handling and Storage Requirements
Precautions for safe handling
Avoid generating and breathing in the dust. Use in a chemical fume hood. Avoid prolonged exposure to the
chemical and contact with the eyes and skin.
Conditions for safe storage
4-Nitrobiphenyl and its solutions must be stored in a secondary container. It must be segregated from other
chemicals that are not classified as select or regulated carcinogens and labeled as a carcinogen.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
4-Nitrobiphenyl
3
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
4-Nitrobiphenyl
4
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)