Standard Operating Procedures
Laboratory Specific
Chemical: alpha-Naphthylamine
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
_____________________________________________________________________________
Department:________________________
Date when SOP was written:_______
Date when SOP was approved by the lab supervisor: ___________________
Principal Investigator:___________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager:___________________________________
Laboratory Phone:____________________ Office Phone:_____________________
Emergency Contact:____________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP:__________________________________________
(Building/Room Number)
_____________________________________________________________________________
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
Crystals of alpha-Naphthylamine are colorless and give off ammonia like odor. Alpha
Naphthylamine is used as a tobacco additive, as a chemical intermediate for certain dyes, in
toning prints and in certain herbicides. The chemical is mostly found as colorless crystals that
darken in air to a reddish purple color, but is also found in solution. Alpha Naphthylamine is
carcinogenic and the target organs are the bladder and skin.
Physical & Chemical Properties/Definition of Chemical Group
CAS#:
134-32-7
Class:
Carcinogen
Molecular formula:
C10H7NH2
Boiling Point:
301°C (573°F) @ 760 mmHg
Melting Point:
50°C (122°F)
1
Potential Hazards/Toxicity
Alpha-Naphthylamine is a carcinogen (known to cause cancer to humans).
 Health Hazards: Avoid direct physical contact. May be fatal or have adverse health
effects if absorbed through skin, inhaled or swallowed.
 Dust and/or vapors can cause irritation to the respiratory tract. May cause allergic skin
reactions, sensitization by skin contact, skin irritation, bladder injury, blood disorders,
and/or nervous system injury.
 Symptoms of exposure include dermatitis, hemorrhagic cystitis, dyspnea (difficulty
breathing), ataxia, methemoglobinema, hematuria (blood in the urine), dysuria.
Personal Protective Equipment (PPE)







Proper personal protective equipment must always be worn while in the regulated area. If
not working under a hood or if working in an open system, a respirator must be worn and
decontamination procedures followed.
This includes wearing a lab coat, safety glasses/goggles, protective gloves, long pants
and closed toe shoes when working with alpha-Naphthylamine (gloves, goggles, lab coat
and respirator).
Wear compatible, chemical-resistant gloves.
Wash and dry hands after working with alpha-Naphthylamine, even if using an isolation
chamber.
Leave lab coats, gloves, and other personal protective equipment in the lab once your
work is complete to prevent the spread of this or other chemicals outside of the lab.
Respirators shall be used to control exposure only in the following circumstances:
o When work is not performed in a fume hood or when the substance is transferred
from one closed system to another.
o During maintenance, repair and other operations for which engineering controls
are not feasible.
o When there is a spill or leak or a closed or isolated system is violated.
o In emergencies such as spills.
Use only respirators and components tested and approved under appropriate
government standards such as NIOSH (US). Where risk assessment shows air-purifying
respirators are appropriate use a full-face particle respirator type N100 (US) or type P3
(EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the
sole means of protection, use a full-face supplied air respirator.
Engineering Controls
Always handle alpha-Naphthylamine in a certified chemical fume hood.
First Aid Procedures



Inhalation or Ingestion: In the case of alpha-Naphthylamine ingestion, obtain medical
attention immediately. If breathed in, move person into fresh air. If not breathing, give
artificial respiration. Consult a physician.
Eye exposure: Immediately flush eyes with copious amounts of water for at least 15
minutes, preferably in an emergency eyewash.
Skin exposures: In the event of skin exposure, remove contaminated clothing and
immediately wash the affected area with soap and copious amounts of water for 15
minutes.
2
Special Handling and Storage Requirements
1. All work with alpha-Naphthylamine is to be done in a " alpha-Naphthylamine" designated
area in order to keep contamination to a minimum. Any persons in this area are required
to wear personal protective equipment. Safety shower and eye wash stations should be
easily accessible where alpha-Naphthylamine is used.
2. All laboratory equipment used in the " alpha-Naphthylamine” designated area are to be
labeled as " alpha-Naphthylamine contaminated" and are not to be removed from the
area without first being decontaminated.
3. Avoid contact with skin and eyes. Avoid formation of dust and aerosols.
4. Provide appropriate exhaust ventilation at places where dust is formed. Normal measures
for preventive fire protection.
5. Keep container tightly closed in a dry and well-ventilated place
6. Light sensitive. Keep in a dry place.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors.
Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small
area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g.,
use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate
personal protective equipment and clean-up material for chemical spilled. Double bag spill waste
in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for
assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in
emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S
at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with
water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor
and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911
(or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center
(emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley
Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF),
x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the
School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center
(emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797
within 8 hours.
3
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash
the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your
extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA
Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be
reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
Waste disposal procedures
1. All alpha-Naphthylamine waste shall be disposed of into waste containers specifically
designated for alpha-Naphthylamine waste.
2. Wash contaminated surfaces to remove any residue.
3. Once the waste container is full, dispose of according the UCLA EH&S hazardous waste
guidelines.
4. Dispose of contaminated packing material as unused product.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
4
Documentation of Training (signature of all users is required)
I have read and understand the content of this SOP:
Name
Signature
5
Date