Transect Instructions for Plant Abundance and Diversity Where there are two or more fieldworkers, you will need to designate one person as the ‘flower visitor observer’ and the others as ‘insect collector for the rest of the data collection – the observer must be the same person so estimations of relative visitor abundance are consistent a. The flower observer will do a trial run of the transect to: b. Use a quadrat (approx. 0.5 m2) to learn approximate size of sample area of flowers to observe Practise going around the transect making observations – if you cannot finish observing all your plants in 1½ hours, note how long it took and check with your trainer for which plants to drop The ‘Near-Instantaneous Count’ method for observations is as follows: Wear dull-coloured clothes, avoid perfume, and stay out of the insects’ flight-path to reduce the effect of your presence First count birds/butterflies over the entire plant Pick sample area of blossoms on the plant that is in sun and has lots of visitors (= maximum data) Approach the sample area quietly, slowly, and do not let your shadow fall on the sample area Count the visitors actually on the flowers, then move closer to count small insects inside flowers If no visitors are present, mark this in the ‘Nil flower visitors’ column Record sun and rain data and any comments using a black pen or dark pencil Move on to next plant as soon as the count is finished and data recorded Count blossom/flower numbers for each plant at some stage each day (use the flower count datasheet) Set up a transect with your trainer on the chosen site with good sun a. Your transect should contain 25–45 plants. Loop the finish back to where you started b. The plants need to be close together (more time counting – less time walking) c. Flowers/blossoms need to be at eye level or lower, or no more than 2 steps up on a stepladder d. Choose flowering plants that are accessible and in sun for at least part of the day e. Tag all plants clearly with a unique ID number made up of your initials and a number, starting at one, e.g. ‘ZW01’ – DO NOT CHANGE THESE – EVER! (See Phase Three for how to add or ‘remove’ plants from a transect as they come in or out of flowering) – if the same plant has been observed before, you need to use the same ID number assigned by the first observer f. If you know the plant’s botanical name you can use it or use an abbreviation such as NVS code – if not, then give the plant a ‘tag’ name that will allow you to find and identify it easily