Chapter 22
Genomics II: Functional Genomics, Proteomics, and Bioinformatics
Study Outline
1. Functional genomics
a. A microarray can identify genes that are transcribed
b. DNA microarrays can be used to identify DNA-protein binding at the genome level
c. Gene knockout collections allow researchers to study gene function at the genomic level
2. Proteomics
a. The proteome is much larger than the genome
b. Two-dimensional gel electrophoresis is used to separate a mixture of cellular proteins
c. Mass spectrometry is used to identify proteins
d. Protein microarrays can be used to study protein expression and function
3. Bioinformatics
a. Sequence files are analyzed by computer programs
b. The scientific community has collected sequence files and stored them in large computer
databases
c. Different computational strategies can identify functional genetic sequences
d. Homologous genes are derived from the same ancestral gene
e. Genetic sequences can be used to predict the structure of RNA and proteins