Introducing Genomics into the High School Classroom
BioPharaceutical Technology Center Institute – BTCI.org
9:00-9:15
9:15-9:55
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Introduction and Overview of Workshop Activities
Genome Sequencing - How it is done.
Sanger Dideoxy Sequencing
454/Ion Torrent
Illumina
PacBio
NanoPore
10:05-11:05 Bioinformatics - Genomes and Genomics: Becoming comfortable wandering
around a genome.
 NCBI: An outstanding, free, online resource for bioinformatics. How do I get
there?
 FASTA file format
 BLAST: What it does, how it does it & understanding its importance to
bioinformatics.
 rDNA: A universal gene set used for identification of organisms. Tree of Life.
 PFAM: Motifs and protein family identification
 Automated Annotation
 Genome Viewer (example: NCBI)
11:15-11:50 Bioinformatics Active Learning
 Hands on: rDNA
 Hands on: BLAST
 Hands on: PFAM
12:00-12:30 Lunch and General Questions
12:30-1:40 Introduction to Online Tools for Genomics Education
12:30-1:20 IMG-ACT and the JGI Toolset - Gene and Pathway Annotation by Students
 How to setup a class.
 How to assign a gene to annotate
 Hands on: Analyze the proC genes.
 Assign a Pathway Analysis
 Hands on: Analyze the Proline Pathway.
1:40-2:50 GENI Project Wet Lab - Functional Genomics
 GENI Collaborative Tool Concept- What GENI can do.
 Hands on: GENI Tour
2:50-3:50 DNA Subway (as time permits)
 Automated Eukaryotic Genome Annotation
 Hands on: Annotate the Zea maize proC gene
 Sequence Alignment and Phylogenetic Analysis
 Hands on: Compare the proC genes of monocots
3:50-4:00 Q & A Wrap-Up
GENI-science.org