There will be a written, assay-type test at the end of the course. The participants will get 50% of their marks from this test (maximum: 100 marks). The remaining
50% will derive from completing short tasks assigned during the laboratory session. There will be 10 assignments each worth 10 marks.
Familiarity with the following study material is strongly recommended. You are not required to purchase the texts listed.
Please note: this is the complete list of required reading material, those papers marked as JC#... will be the papers for discussion in each of the Journal Clubs we will have along the workshop, 4 in total.
All the others are for review.
LIST OF PARPERS
REFERENCES
Web based training
( http://micro.magnet.fsu.edu/primer/virtual/virtual.html
).
Text: “FISH: A practical approach. “ Eds.Beatty,B., Mai,S., Squire,J. Oxford
University Press. 2002.
-An Introduction to the Optical Microscope, Revised Edition
By: Savile Bradbury St. Edmundsbury Press © 1989, 86 pgs., illustrated
-Introduction to Light Microscopy (Microscopy Handbooks, No 42)
Authors: Savile Bradbury and Brian Bracegirdle ©1998, 123 pages.
-Light Microscopy in Biology: A Practical Approach
Edited by: A. J. Lacey IRL Press ©1989, 327 pages, illustrated.
-Optimizing Light Microscopy for Biological and Clinical Laboratories
Author: Barbara Foster Kendall/Hunt © 1997, 187 pages, illustrated, paperback
JC# 1a
Wilkens L, Flemming P, Gebel M, Bleck J, Terkamp C, Wingen L, Kreipe H,
Schlegelberger B. Induction of aneuploidy by increasing chromosomal instability during dedifferentiation of hepatocellular carcinoma. PNAS vol 101, number 5, 1309-1314
(2003)

JC# 1b
Kim HJ, Woo HY, Koo HH, Talk EY, Kim SH. ABL Oncogene amplification with p16
INK4a Gene deletion in precursor T-cell Acute Lympoblastic Leukemia/Lymphoma:
Report of the first case. American Journal of Hematology 76: 360-363 (2004)
References on MACS :
1) A user report with µMACS Protein A MicroBeads showing the procedure of immunopurification with MACS Technology.
<<user_report_SchwarzMerling.pdf>>
2) The µMACS Epitope Tagged Protein Isolation Kits user manual including an introduction to protein isolation via epitope tags (section 1., Description).
References on PRINS :
-Koch JE, Kolvraa S, Petersen KB, Gregersen N, Bolund L.
Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma 1989; 98: 259-65.
-Kadandale JS, Wachtel SS, Tunca Y, Martens PR, Wilroy RS, Tharapel AT.
Deletion of RBM and DAZ in azoospermia: Evaluation by PRINS. Am J Med Genet
2002; 107: 105-8.
-Cinti C, Stuppia L, Maraldi NM. Combined use of PRINS and FISH in the study of the dystrophin gene. Am J Med Genet 2002; 107: 115-8.
JC# 2a
-Tharapel AT, Kadandale JS, Martens PR, Wachtel SS, Wilroy RS. Prader
Willi/Angelman and DiGeorge/Velocardiofacial syndrome deletions: Diagnosis by Primed In Situ Labeling (PRINS). Am J Med Genet 2002; 107: 119-22.
JC# 2b
-Tharapel SA, Kadandale JS. Primed in situ labeling (PRINS) for evaluation of gene deletions in cancer. Am J Med Genet 2002; 107: 123-6.
-Coullin P, Roy L, Pellestor F, et al. PRINS, the other in situ labeling method useful in cellular biology. Am J Med Genet 2002; 107: 127-35.
-Yan J, Bronsard M, Drouin R. Creating a new color by omission of 3 end blocking step for simultaneous detection of different chromosomes in multi-PRINS technique. Chromosoma 2001; 109: 565-70.
JC# 3a
-Yan J, Zhang XX, Fetni R, Drouin R. Trisomy 8 and monosomy 7 detected in bone marrow using primed in situ labeling, fluorescence in situ hybridization, and conventional cytogenetic analyses. A study of 54 cases with hematological disorders. Cancer Genet Cytogenet 2001; 125: 30-40.

JC# 3b
-Pellestor F, Quenesson I, Coignet L, et al. FISH and PRINS, a strategy for rapid chromosome screening: application to the assessment of aneuploidy in human sperm. Cytogenet Cell Genet 1996; 72: 34-6.
-Metasystems XCyte Manual
-Metafer – a Novel Ultra High Throughput Scanning System
JC# 4a
Metafer a Novel Ultra High Throughput Scanning System for Rare Cell Detection and
Automatic Interphase FISH Scoring.Macek M Sr, Bianchi D, Cuckle H (eds): Early
Prenatal Diagnosis, Fetal Cells and DNA in the Mother, Present State and Perspectives.
12th Fetal Cell Workshop, Prague, May 2001. pp 329 - 339
JC# 4b
-A reference on mBAND, a special FISH analysis technique: I. Chudoba, A. Plesch, T.
Lörch, J. Lemke, U. Claussen, G. Senger :High resolution multicolor-banding:a new technique for refined FISH analysis of human chromosomes. Cytogenetics and Cell
Genetics, 84, pp 156-160.