Plant Science 446/546 Class Test February 18, 1998 9:30 to 10:20 Ag.Sci Room 339 Name : Answer all 8 questions A bonus question is available for an extra 5 points A total of 100 points are available Points available from each part of each question are shown in bold square parenthesis Try to be as brief and concise as possible Please write in a legible form Show any working/calculations Make sure that any additional paper used 1 is attached to the questionnaire 2 1a. The transition of mankind from a hunter/gatherer to a farmer involved: (1) domestication of livestock, and (2) domestication of crops. Which (livestock or crops) did humans domesticate first? Explain your answer [10 points]. 1b. A combination of natural selection and selection directed by plant breeders (early and modern), has influenced the crops we now grow. List five characters that mankind has selected for which would not have been selected by a natural evolution process [5 points]. 1. 2. 3. 4. 5. 2a. In order to produce a hybrid cultivar using cytoplasmic male sterility you require three types of genotypes. Describe the difference between the three types required [4 points]. 2b. You are to embark on a backcrossing study designed towards convergence breeding. You have at your disposal two genotypes coded C and M. Genotype C is a cytoplasmic male sterile line, but one that does not have very good hybrid combining ability; genotype M has great hybrid combining ability and is male fertile (without a male fertile restorer gene). Design a scheme (listing each operation necessary) that will result in a genotype which is 95% the genotype of the M line but is cytoplasmic male sterile [6 points]. 3a. Briefly outline the main features of a crop species that would allow you to design a breeding program to develop a Hybrid, synthetic, multiline, pure-line and clonal cultivar. List one species which would be appropriate to develop cultivars of each type [20 points]: a. A hybrid cultivar Features: Suitable crop species: b. a synthetic cultivar Features: Suitable crop species: 3 c. a multiline cultivar Features: Suitable crop species: d. A pure-line cultivar Features: Suitable crop species: e. A clonal cultivar Features: Suitable crop species: 4a. 4c. Explain the main features of the two mechanisms for plant resistance in plant breeding [6 points]. a. Resistance to inhibition of infection: b. Resistance to inhibition of subsequent growth after infection: Four single, dominant, genes (Coded, A, B, C, and D) have been identified each which offers complete immunity to a specific race of powdery mildew. Virulence genes have also been identified in the pest (a, b, c, and d). Given the information below, indicate whether each genotype would be resistant or susceptible to the disease [4 points]. Genotype 1. Genotype 2. Genotype 2. Genotype 3. 5a. Genotype of the plant AaBBccdd aabbccDD AabbCCDd AABBCCDD Genotype of the pest aaBbCCdd AAbbCCdd aaBBccdd aabbccdd Plant response You have recently been appointed as consultant to a large plant breeding company with a whopping great salary, great benefits and wonderful pension fund (aren't you glad that you did this course now?). The company wants to develop a breeding program to develop superior peach cultivars. Outline five points you will consider before setting your breeding objectives [5 points]. 1. 2. 4 3. 4. 5. 5b. Briefly indicate factors you would consider if asked to design a taste test to determine which of your top three peach breeding lines had best organoleptic characters [5 points]. 6. Describe the difference between a pedigree breeding scheme, a bulk breeding scheme, and a pedigree/bulk breeding scheme, and outline the advantages and disadvantages that each breeding scheme would offer for developing superior spring wheat cultivars [12 points]. 7a. List five different plant parts that can be responsible for asexual reproduction [5 points]. 1. 2. 3. 4. 5. 7b. List three disadvantages associated with breeding clonal crops [3 points]. 1. 2. 3. 7b. Mass selection and recurrent phenotypic selection are often used in developing outpollinating cultivars. Describe the main difference between the selection methods and indicate any problems that each selection method could have in a breeding program [5 points]. 8. You are walking along a deserted beach in Southern California (are there any of these left) and you inadvertently kick a brass oil lamp. On picking up the lamp you gently rub the side and - poof! - out appears a 20 foot tall genie. "Oh master!" says the genie," I can grant you two wishes" (well times are hard and the old three wished thing does not apply any more). You think for a few minutes then say "I would like you to conjure me up the most wonderful plant that exists within the Universe, so that I can grow it on my farm in Idaho and make millions of dollars in profit". Poof! this plant appears before you. A plant species that has never appeared on earth before. 5 As a plant breeder, list five questions you would ask the genie about the biology of this plant that would help you design a cultivar development program to increase the value of it's crops [5 points]. 1. 2. 3. 4. 5. Having sorted that out, you begin to think of your other wish. "I would like you to tell me the formula for a chemical apomict" (a chemical, when applied to a crop will result in 100% apomictic seed from the crop). "Well" says the genie. "I can do this, but, you must specify which crop species the chemical will work on (it can only work on one chosen species)". What crop species would you chose to have apomictic seeds, and why? [5 points]. Bonus Question What is the most likely center of origin of the following crop species [5 bonus points]. Species Brassica napus, Canola Sorghum bicolor, Sorghum Musa, Banana Glycyrhiza glabra, Liquorice Helianthus, Sunflower Center of origin - 6