Breeding Addendum Animal Research Ethics Board – McMaster University Principal Investigator - AUP# Who will be doing breeding? Select applicable *If the researcher is doing the breeding, quarterly breeding numbers must be submitted to the Animal Facility, to ensure accuracy of numbers used for yearly census reporting to the Canadian Council on Animal Care and the Ontario Ministry of Agriculture, Food and Rural Affairs. Are they outbred? If no, complete below. 1) Yes Inbred, Transgenics, Knock-out/Knock-In Strains (complete nomenclature) 2) 3) 4) No Cre-Flox End Experimental Genotype (complete nomenclature) Inbred (B x S) Controls (complete nomenclature) Breeding System Monogamous *If Harem, provide justification. Triples Tissue Specific? Transgenics/Knock-Outs Is Genotyping required? If yes, provide the following: Tissue Genotyped All-Body Knock-Out Tissue Type Observed (plugs) Congenic (B x S) Harem* Yes Age at Genotype SOP# Followed Anaesthetic Used Induced Knock-Out No Analgesic Used Revised November 2015