Course Name: Instrumental Analysis II
Course Code: 700425
Credit Hours: 3 Hrs (2 Lec. + 1 Prac)
Course Description
This course aims to introduce to the students a concept of applying the available instruments used for separation of mixtures as well as qualitative and quantitative analysis of medicinal and pharmaceutical formulated medicines. The course covers different chromatographic methods and techniques ( PC,TLC, IEC, CC, GPC,GC,
HPLC) inn addition to nuclear magnetic resonance and mass spectroscopy.
Course objectives:

To learn basic principles governing chromatographic separation techniques.

To explore applications of chromatographic methods used in pharmaceutical compounds, dosage forms and drugs and their metabolites in biological fluids.

To develop basic skills in prepare and operate chromatographic equipment.

To learn to apply basic principles in order to develop a chromatographic method to achieve a particular objective.

To learn the basic principles and the applications of I. R, NMR and Mass spectroscopy.

To improve skills in observation, keeping records, and interpreting data by using the mentioned techniques. .
1.
Course contents:
Introduction to separations techniques using chromatography .
Purpose of separations, other different techniques of separation. (1
lecture)
2.
Sample preparation methods . Considerations in sample preparation. (2 Lectures)
3. Basic theoretical considerations and terminology of
chromatography . Theory of chromatography, classification, application, stationary and mobile phases, quantitative and
qualitative analysis. ( 4 Lectures)
4. Planar chromatography. Paper and thin layer chromatography .
Theory, practical considerations and pharmaceutical applications ( 2
Lectures)
1

5. Gas chromatography . Theory, practical considerations. And
pharmaceutical applications (2 lectures):
6. Open column liquid chromatography . Theory, practical
considerations and pharmaceutical applications (2 lectures):
7. High performance liquid chromatography . Theory, practical
considerations and pharmaceutical applications (3 lectures)
8. Nuclear Magnetic resonance spectroscopy . Theory, practical
considerations and pharmaceutical applications (5 lectures)
9.
Mass Spectroscopy . Theory, practical considerations and
pharmaceutical applications (4 lectures):
10. Interpretation of combined spectra . ( UV, IR, NMR ) for certain
drugs and pharmaceutical compounds (6 lectures)
Methods of instruction
1.
Lectures.
2.
Computer assisted sessions.
3.
Practicals.
4.
Tutorials.
Practical:
1.
Preparation of paper chromatography ( spotting , visualizing the
spots and determination the Rf value)
2.
Use of thin layer chromatography ( spotting , visualizing the spots
and determination the Rf value) .
3.
Two dimensional PC and TLC.
4.
Preparative PC and TlC
5.
Test on different techniques of PC and TLC.
6.
Column chromatography 9separation of dyes and plant pigments)
7.
Gas chromatography, basics ( demonstration using quick time movies
of GC)
8.
Gas chromatography, individual labs ( result interpretation)
9.
HPLC, Demonstration and analysis of certain examples of drugs.
10. NMR , Mass spectroscopy : Interpretation of combined spectra for
certain drugs and pharmaceutical compounds.
2

Main Text Book :
1. Spectroscopic methods in organic chemistry, By Williams D.H. and Fleming Pub.
McGraw Hill, Berkshire, 1997
2. Chromatographic methods by Stock R. and Rice C.B.F., Pub. Chapman and Hall,
London 1987
Additional Reference:
1. Pharmaceutical Analysis, By David G. Watson, Pub. Churchill Livingstone,
Edinburgh 1999
2. Fundamentals of Analytical Chemistry, By Skoog, West and Holler, 7 th
edition,
Saunders College Publishing, 1997
3. Pharmaceutical Analysis (Instrumental Methods) vol. II, By A.V. Kasture,
K.P.Mahadik, S.G. Wadodkar and H.N. More, Pub. Niralli Prakashan, Buchwar,
1995
Journals
1. Journal of Chromatography.
2. Journal of Liquid Chromatography.
3. .Journal of Magnetic Resonance.
4. Journal of the American Society for Mass Spectrometry
(JASMS)
Pre-requisites-course code
1. Instrumental Analysis I course code 700422
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