Protocol #:______________ UTSA ANIMAL-USE PROTOCOL APPLICATION Appendix H: Breeding of Research Animals P.I. : 1. Justification for in-house breeding: Could the animals that will be bred be purchased from commercial sources in the required number? No. The animals are not available commercially in sufficient number. Yes. The animals are available commercially. Please describe the rationale for breeding these animals at UTSA: 2. Please identify the source of breeders: Colony managed by a UTSA Investigator. Specify PI’s name & source protocol#: Obtained from another institution or non-UTSA investigator. Identify source: Purchased from a LARC-approved vendor (click here for list of approved vendors) Identify vendor: 3. Select all that apply: Breeding scheme will be monogamous, non-continuous scheme. Breeding scheme will be OTHER THAN monogamous, non-continuous scheme (see IACP Policy 013) Justify: Please list all strains for which each of the above is the case: 4. Select all that apply: Offspring will be weaned at 18-21 days of age. Offspring will be weaned at times other than 18-21 days of age. Justify: Please list all strains for which each of the above is the case: 5. Special care (feed, water, temp, humidity, air flow) required for this breeding colony: No special care is required. Special care is necessary to keep these animals healthy. The required special care is as follows (describe): (Appendix H Ver. 01/30/14) Page 1 of 2 Protocol #:______________ Breeding Colony Species and Numbers: Species Number of male breeders Number of female breeders Expected number of offspring Estimated # of offspring needed for this protocol Estimated # of offspring transferred to another protocol Estimated # of offspring euthanized without use Copy & Paste the Table above and Section 6 for each additional species. 6. Disposition of Breeders & Unneeded Offspring: Indicate the final disposition of retired breeders: Euthanasia according to protocol Used in this protocol, describe below. Other, such as transfer to another protocol, describe below. Please describe any planned experimental use and/or other disposition: Clarification of the final disposition of offspring that are not required for this protocol: If transfer to another protocol is indicated in section Q2 above, please specify the UTSA protocol number and identify the Principal Investigator. In the event that transfer is to a non-UTSA investigator, please explain the circumstances of the transfer. If euthanasia without use is indicated in section Q2 above, please explain why the surplus offspring cannot be used for this protocol or by another investigator. (Appendix H Ver. 01/30/14) Page 2 of 2