Eotaxin (murine)
PRODUCT INFORMATION
Description
Eotaxin (EOT) is a member of the C-C, or β chemokine class. Eotaxin was originally
isolated from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol
challenge with ovalbumin. EOT is an approximately 8.4 kDa polypeptide of 74 amino
acids. The precursor form of eotaxin consists of 97 amino acids. To generate the
mature eotaxin (74 amino acids), a 23 amino-terminal amino acid signal peptide is
cleaved from the precursor. EOT is chemotactic for eosinophils, but not mononuclear
cells or neutrophils. Human EOT shows approximately 60% amino acid homology to
mouse and guinea pig eotoxin. Eotaxin also shows high identity with MCP-1, 2, and 3.
Recombinant Murine Eotaxin produced in E. coli is a single, non-glycosylated
polypeptide chain containing 74 amino acids and having a molecular mass of 8,403.2
Dalton. The rmEotaxin is purified by proprietary chromatographic techniques.
Cat. No.: Z00302
Size: 1 mg or 2 ug or 10 ug
Source: Escherichia coli.
MW: 8,403.2 Dalton
Endotoxin Level: Less than
0.1 ng/μg (IEU/μg) of
rmEotaxin.
Dimers and aggregates:
Less than 1% as determined
by silver-stained SDS-PAGE
gel analysis.
Version: 08/24/2006
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Quantitation
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of mEotaxin as a Reference
Standard.
Specific Activity
The Biological activity is determined by its ability to induce chemotaxis purified
eosinophils at a concentration of 100-1,000 ng/ml.
Storage
Lyophilized rmEotaxin although stable at room temperature for 3 weeks, should be
stored desiccated below -18 °C. Upon reconstitution rmEotaxin should be stored at
4 °C between 2-7 days and for future use below -18 °C. For long term storage it is
recommended to add a carrier protein (0.1% HSA or BSA). Please prevent
freeze-thaw cycles.
Formulation
Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Reconstitution
It is recommended to reconstitute the lyophilized rmEotaxin in sterile 18 MΩ-cm H2O not
less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Sequence
HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC
ADPKKKWVQD ATKHLDQKLQ TPKP
Sequence analysis
The sequence of the first five N-terminal amino acids was determined and was found to
be His-Pro-Gly-Ser-Ile.
** For non-clinical research use only. **
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