Fig. 1S: Validation of RT-qPCR assay. Samples from the population survey in July were used
for assays with different pairs of primers (n = 78). Results of Pearson’s correlation of primer
pairs E1 against E2 and E1 against E3 are both r = 0.99, P < 0.001. For primer information see
Table S4.
Fig. 2S: Predicted amino acid sequence of PgβGLU-1 protein. A 25 amino acids
hydrophobic signal peptide was identified in N-terminal (underlined amino acids) and cleavage
site predicted between amino between amino acids 25 and 26, in black box (SignalP 4.1 server).
Conserved amino acid residues that are part of the active site and interact with the glycone
meoity are boxed (Q49, H152, N198, W462, E469, W470).
Table S1. Differentially expressed genes. Gene information and annotations are as described in
Rigault et al. 2011. Fold change is based on ratios between R and N-R
Table S2. Variations in Pgβglu-1 amino acid sequence of resistant (R) and non-resistant (N-R)
trees. Sites are considered variable if two or more variants are observed among the 14 sequences.
Table S3. White spruce provenances sampled for the population survey
Table S4. Primer sequences used for quantitative PCR analysis, molecular cloning, and
sequencing assays