Cat. No.: Z00374
Size: 1 mg or 10 ug or 2 ug
Source: Chinese Hamster
Ovary Cells
MW: 18,606.29 Dalton
IL-10 (human)
PRODUCT INFORMATION
Description
Recombinant Human IL-10 produced in CHO is a single, glycosylated polypeptide chain
containing 161 amino acids and having a molecular mass of 18,606.29 Dalton. The
rHuIL-10 is purified by proprietary chromatographic techniques.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Endotoxin Level
Less than 0.1 ng/μg (IEU/μg) of rHuIL-10.
Quantitation
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of IL-10 as a Reference Standard.
Specific Activity
The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of
MC-9 cells was found to be < 2 ng/ml, corresponding to a Specific Activity of 1.5 x 10 7
IU/mg.
Storage
Lyophilized rHuIL-10 although stable at room temperature for 3 weeks, should be stored
desiccated below -18 °C. Upon reconstitution rHuIL-10 should be stored at 4 °C
between 2-7 days and for future use below -18 °C. For long term storage it is
recommended to add a carrier protein (0.1% HSA or BSA). Please prevent
freeze-thaw cycles.
Formulation
The protein was lyophilized from a concentrated (1 mg/ml) solution containing 60mM
PBS pH=6.5 and 100 mM NaCl,
Reconstitution
It is recommended to reconstitute the lyophilized rHuIL-10 in sterile 18MΩ-cm H2O not
less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Sequence
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK
ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK
TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY
IEAYMTMKIR N
Dimers and aggregates
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Sequence analysis
The sequence of the first five N-terminal amino acids was determined and was found to
be Ser-Pro-Gly-Gln-Thr.
** For non-clinical research use only. **
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