Name _______________________________ Unit 1 Review Sheet 1.1 Branches of Science o biology o zoology o botany o taxonomy o entomology o herpetology o mammalogy o ornithology 6 themes of biology o cell unicellular multicellular DNA o homeostasis o reproduction/ inheritance/ gene asexual sexual o evolution natural selection adaptation o interdependence ecology ecosystem o need for energy photosynthesis autotrophs heterotrophs Per. ______ 1.2 Characteristics of Life cell organization metabolism cell division development 1.3 Scientific Method problem research hypothesis experiment observation conclusion communicating 1.4 Microscopy and Measurement Janssen Brothers Leeuwenhoek light microscope o parts of microscope (diagram & use) o magnification calculations o resolution o diameter of field o inversion of image o micrometers measuring size of object electron microscopes o TEM o SEM ultra centrifuge chromatography electrophoresis computers 1 Unit 1 The Science of Life first life arose on earth 3.5 billion years ago 1-1 Study of life = BIOLOGY content so vast that specialties have been developed: o botany study of plants o zoology study of animals o anatomy study of plant and animal parts o taxonomy study of classification o cytology study of cells o genetics study of genetics, heredity & DNA o physiology study of how things work o entomology study of insects o herpetology study of amphibians and reptiles o mammalogy study of mammals o ornithology study of birds other sciences important to biology: = chemistry and physics 2 botany/ anatomy herpetology cytology genetics ornithology entomology Biology unified by six themes: 1. Cell Structure and Function all organisms are made of cells the cell is the basic unit of life unicellular = one cell multicellular= many cells all surrounded by a membrane and enclosing genetic material (DNA) 2. Stability and Homeostasis all living things maintain a stable internal environment (= homeostasis) o stimulus = anything that causes an organism to react o response = reaction to the stimulus 3. Reproduction and Inheritance reproduction= production of new offspring DNA= nucleic acid, double helix shaped gene= segment of DNA, unit of hereditary info all transmit hereditary information asexual reproduction= one parent, without egg or sperm sexual reproduction= two parents, combination of egg & sperm 4. Evolution describes changes in populations of organisms over time natural selection= favorable variations reproduce faster adaptation= increases an organism’s chance at survival 5. Interdependence of Organisms interactions of organisms with each other and their environment ecology= study of relationship between organisms & their environment ecosystems= biotic (living) & abiotic (non-living) component of environment 6. Matter, Energy and Organization organisms require constant supply of energy, the origin of which is the sun photosynthesis= light energy converted to chemical energy autotrophs= make their own food heterotrophs= need to obtain food 3 Regents Questions: 1. The arrows in the diagram below indicate the movement of materials into and out of a singlecelled organism. 4. The diagram below represents possible evolutionary relationships between groups of organisms. The movements indicated by all the arrows are directly involved in (1) the maintenance of homeostasis (2) photosynthesis, only (3) excretion, only (4) the digestion of minerals 2. Which process usually results in offspring that exhibit new genetic variations? Which statement is a valid conclusion that can be drawn from the diagram? (1) Snails appeared on Earth before corals. (2) Sponges were the last new species to appear on Earth. (3) Earthworms and sea stars have a common ancestor. (4) Insects are more complex than mammals. 5. The diagram below represents a food web. 3. A food web is represented in the diagram below. Which population in this food web would most likely be negatively affected by an increase in the mouse population? (1) snake (3) wolf (2) rabbit (4) hawk 4 Two of the herbivores represented in this food web are (1) toads and snakes (2) deer and mice (3) wolves and raccoons (4) grasshoppers and toads 1-2 Characteristics of Life cells all living things composed of cells all living things are highly organized at the cell and molecular level all living things use energy tissues organs metabolism= sum of all chemical processes in living things all living things maintain stable internal environments organ system homeostasis= stable internal environment organism all living things grow cell division= making more number of cells development= process by which organisms grow all species of organisms can reproduce Which term best describes each image? 1. cell division 2. tissue development 3. homeostasis 5 Regents Questions: 1. Normally, when the concentration of glucose in the blood falls below a certain level, stored glucose reenters the blood until the original concentration is reached again. This regulation of the concentration of blood glucose is part of the process known as (1) synthesis (3) pinocytosis (2) respiration (4) homeostasis 2. The addition of new cells and corresponding increase in organism size is known as (1) growth (3) regulation (2) transport (4) respiration 3. Which term is defined as all the chemical reactions that are required to sustain life? (1) metabolism (3) nutrition (2) regulation (4) synthesis 4. The ability of the human body to maintain a constant body temperature is an example of (1) transport (3) homeostasis (2) metabolism (4) synthesis 5. Cells are to tissues as organs are to (cells tissues organs organ systems organism) (1) organ systems (2) cells (3) genes (4) organelles 6. A biologist would most likely study all of the chemical activities of an organism to obtain information about the organism’s (1) number of mutations (3) development (2) reproductive cycle (4) metabolism 7. Which sequence is listed in order from simplest to most complex? (1) tissue cell organ system organ (2) cell tissue organ organ system (3) cell tissue organism organ (4) organism tissue organ organ system 6 1-3 Scientific Methods many forms, but most similar: 1. define problem/ ask question 2. collect data = observing, measuring, sampling, organizing data 3. form hypothesis = suggested explanation that is testable predicting= an educated guess 4. experimenting = testing a hypothesis by gathering data under controlled conditions control group= serves as a comparison experimental group= the condition varied single variable= independent variable/ dependent variable 5. drawing conclusions what you change/ what you measure producing a model explanation supported by data inference= a conclusion based on facts rather than direct observations 6. communicating report research results (where?) hypothesis continuously supported by research is termed a theory 7 Name: ______________________________________ Critical Thinking / Problem Solving Activity: Per. _________ Can you Spot the Scientific Method? Each sentence below describes a step in the scientific method. Math each sentence with a step of the scientific method listed below: A. recognize a problem C. test the hypothesis with an experiment B. form a hypothesis D. draw conclusions __B__ 1. Stephen predicted that seeds would start to grow faster if an electric current traveled through the soil in which they were planned. __B__ 2. Susan said, “If I fertilize my geranium plants, then they will blossom.” __D__ 3. Jonathan’s data showed that household cockroaches moved away from raw cucumber slices. __C__ 4. Rene grew bacteria from the mouth on special plates in the laboratory. She placed drops of different mouthwashes on bacteria on each plate. __C__ 5. Kathy used a survey to determine how many of her classmates were left-handed and how many were right-handed. __A__ 6. Dana wanted to know how synthetic fibers were different from natural fibers. __A__ 7. Jose saw bats catching insects after dark. He asked, “How do bats find the insects in the dark?” __A__ 8. Justin wondered if dyes could be taken out of plant leaves, flowers, and stems. __C__ 9. Jenny soaked six different kinds of seeds in water for 24 hours. Then she planted the seeds in soil to a depth of 1cm. She used the same amount of water, light, and heat for each kind of seed. __A__10. Bob read about growing plants in water. He wanted to know how plants could grow without soil. __B__11. Kevin said, “If I grow five seedlings in red light, I think the plants will grow faster than the five plants grown in white light.” __D__12. Angela’s experiment proved that earthworms moved away from light. __B__13. Scott said, “If acid rain affects plants in a particular lake, it might affect small animals, such as crayfish, that live in the same water.” __D__14. Mike fed different diets to three groups of guinea pigs. His experiment showed that guinea pigs need vitamin C and protein in their diets. __D__15. Kim’s experiment showed that chicken eggshells were stronger when she gave the hen feed to which extra calcium had been added. 8 Name: _________________________________________ The Scientific Method Put the following steps of the scientific method in the proper order. Per. _______ __6___ Organize and analyze data __3___ State a hypothesis __1___ Identify the problem __7___ State conclusion __4___ Design and carry out an experiment __5___ Make observations and record data __2___ Gather information Math the term in Column I with its definition in Column II. Column I 1. theory ___F___ 2. law ___G___ 3. hypothesis ___A___ 4. experiment ___B___ 5. variable ___C___ 6. control ___E___ 7. data ___D__ 8. conclusion ___I___ 9. application ___H___ Column II a. suggested explanation to a problem or observation based upon known information b. used to test a hypothesis c. anything that can affect the results of an experiment d. observations and measurements made during an experiment e. part within the experiment that is maintained without change in order to provide a comparison for the part of the experiment containing the variable f. Hypothesis that has been tested and supported by a great amount of evidence over a long period of time g. Statement describing (but not explaining) a natural event or phenomenon h. New use to which results are put or new technique developed i. A summary that explains whether or not the data support the hypothesis Regents Questions: 9 1. An experiment was performed to determine the effect of different mineral salts on plant growth. Forty pots containing genetically identical plants were divided into four equal groups and placed in a welllighted greenhouse. Each pot contained an equal amount of non-mineral potting soil and one plant. Minerals were then added in equal amounts to each experimental group of pots as shown below. For the experiment to be valid, what should be added to the control group of pots? (1) water (3) potassium salts (2) nitrogen salts (4) potassium and phosphorus salts 2. The diagram below illustrates the result of growing a garlic bulb in a cup of distilled water over five days. Design an experiment consisting of a control and three different experimental groups to test the prediction, “Garlic grows better as the salt concentration of the solution in which it is grown increases.” In your answer, be sure to: • describe the control to be used in the experiment [1] • describe the difference between the three experimental groups [1] • state one type of measurement that should be made to determine if the prediction is accurate [1] • describe one example of experimental results that would support the prediction [1] __*garlic in water with no salt__________________________________________________________________ __*different amount of salt in water___________________________________________________________ __*leaf length, root length______________________________________________________________________ __*the garlic bulb grown with the most amount of salt had the longest leaves; the garlic grown with the most amount of salt had the longest roots____________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ 10 3. In an investigation designed to determine the effect of the amount of water on plant growth, two groups of equal-sized bean plants of the same species were grown under identical conditions, except for the amount of water they were given. One group was watered with 200 milliliters of water once a day, while the other group was watered with 400 milliliters of water once a day. After several days, the heights of the plants were measured. It was determined that the plants watered with 400 milliliters of water once a day showed more growth. The variable in this investigation is the __amount of water__________________. 4. A new drug for the treatment of asthma is tested on 100 people. The people are evenly divided into two groups. One group is given the drug, and the other group is given a glucose pill. The group that is given the glucose pill serves as the __control___________________. The group that is given the drug serves as the ___experimental__________________. 5. A student is designing a procedure to determine the effect of the absence of a specific amino acid in the nutrient culture medium of a certain species of bacteria. Using one of more complete sentences, describe the control that the student should use in the experiment. __bacteria grown in a nutrient culture medium WITH the amino acids_____________________________________________________________________________________________ __________________________________________________________________________________________________ 6. You are the head of the research division of the Leafy Lettuce Company. Your company is experimenting with growing lettuce using hydroponic technology. Hydroponic technology involves growing plants in containers of growth solution in a greenhouse. No soil is used. The growth solution that the company uses contains water, nitrogen, and phosphorus. The company wants to know if adding iron to this formula will improve lettuce growth. Briefly describe how to test the effect of the formula with iron added. In your description, be sure to: a) b) c) d) State a hypothesis to be tested in the new experiment State how the control group will be treated differently from the experimental group. Identify two factors that must be kept the same in both the experimental and control groups. State what types of data should be collected to support or refute the hypothesis. a) If I add iron to the growth solution, then lettuce will grow better. b) Control will not have iron added to the growth solution, the experimental group will have iron added to the growth solution. c) Same species of lettuce; same growth solution of water, nitrogen, and phosphorus; same amount of light; same amount of days; etc. d) height of lettuce; width of lettuce; growth rate; number of leaves; etc. (Do NOT write “growth”... you have to be able to measure. 11 __A_ 7. Which condition is necessary for an experiment to yield useful data? (a) similar results should be obtained when the experiment if repeated (b) only the expected results should be considered each time experiment is performed (c) the hypothesis is correct (d) the experimental period must be short _A__ 8. An organism was kept at a temperature of 40 oC for a period of 2 weeks. At the end of that time, the investigator determined that the organism was sterile. To support the hypothesis that high temperatures cause sterility, the investigator should be able to show that the (a) organism was not sterile before the experimental period began (b) high temperature did not alter the blood pressure of the organism (c) pituitary gland, which controls sterility, of the organism had not degenerated (d) organism was homozygous for temperature sensitivity __C_ 9. A nutrient medium was prepared by mixing powdered agar with boiling distilled water. Some of the prepared medium was then placed in a sterile petri dish, covered, and allowed to solidify. The cover was then removed and the agar was touched to a door knob. The petri dish was covered again and incubated at 37 oC. After 48 hours, bacterial growth was observed. The investigator concluded that bacteria on doorknobs cause disease. One error was that the investigator (a) used distilled water (b) covered the petri dish (c) did not show that bacteria caused disease (d) did not incubate the materials at the proper temperature __B_ 10. A student conducted an original, well-designed experiment, carefully following proper scientific procedure. In order for the conclusions to become generally accepted, the experiment must (a) contain several experimental variables (b) be repeated to verify the reliability of the data (c) support the original hypothesis (d) be conducted by a scientist _D_ 11. In a controlled experiment, 20 marigold plants of the same age were grown singly in 20 different pots containing soil of the same composition and moisture level. The pots were divided into two groups of 10. One group was exposed to 8 hours of sunlight each day for 15 days, and the other group was exposed to 8 hours of light from a 75-watt bulb for the same period. In this investigation, the source of light represents the experimental (a) problem 12 (b) control (c) hypothesis (d) variable _D__ 12. Which experimental procedure would best determine the effectiveness of a vaccine for preventing a certain disease in pigeons? (a) Expose 100 pigeons to the disease and then inoculate all 100 pigeons with the vaccine. (b) Expose 100 pigeons to the disease and then inoculate 50 of these pigeons for this disease. (c) Inoculate 10 pigeons with the vaccine and 90 pigeons with a harmless solution and then expose all 100 pigeons to the disease. (d) Inoculate 50 pigeons with the vaccine and 50 pigeons with a harmless solution and then expose all 100 pigeons to the disease. A student conducting an experiment placed five geranium plants of equal size in environmental chambers. Growing conditions were the same for each plant except that each chamber was illuminated by a different color of light of the same intensity. At the end of 20 days, plant growth was measured. 13. Using one or more complete sentences, state a possible hypothesis for this experiment. __If I grow a plant under red light, then the plant will grow faster. ________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ 14. Using one or more complete sentences, state the control that should be used in this experiment. __Geranium plants grown under sunlight.________________________________________________________ __________________________________________________________________________________________________ __________________________________________________________________________________________________ A sterile petri dish, A, containing sterile nutrient agar was exposed to the air for a few minutes. A second sterile petri dish, B, containing sterile nutrient agar was not opened. Both petri dishes were placed in a warm, dark place. Three days later, petri dish A was observed to contain a population of bacteria, which petri dish B had no bacterial growth. 15. Using one or more complete sentences, explain how petri dish B serves as a control for the experiment. __Petri dish B was not opened, meaning the bacteria did not grow from the agar. _________ _________________________________________________________________________________________________ _________________________________________________________________________________________________ 16. Using one or more complete sentences, state a hypothesis that this experiment could have been testing using petri dish B as the control. __If I open a petri dish, then bacteria will grow.___________________________________________ ________________________________________________________________________________________________ ________________________________________________________________________________________________ 13 17. A scientist performed an experiment using the following steps: Define and research the problem X Set up and Make conduct the observations experiment once and record data Formulate a conclusion Repeat the experiment Using one or more complete sentences, identify the step that belongs in box X. __state a hypothesis. __________________________________________________________________________ Base your answers to questions 18 through 20 on the information and diagram below. An investigation was carried out using the two setups shown below. Other than the difference shown in the diagram, all other conditions were identical. 18. State one possible hypothesis that could be tested using these setups. __If I decrease the temperature, then plants will grow taller.______________________________ _________________________________________________________________________________________________ _________________________________________________________________________________________________ 19. What data should be collected in order to test the hypothesis stated in question 18? __Height of plant_______________________________________________________________________________ _________________________________________________________________________________________________ _________________________________________________________________________________________________ 20. Describe one change that could be made in the investigation to improve it. __Increase the temperature difference; _____________________________________________________ Use more plants for each setting (More sample size, more validity.)________________________ _________________________________________________________________________________________________ 14 1-4 Microscopy and Measurement Microscopes 1590 Janssen Brothers o first compound microscope van Leeuwenhoek o fish tail capillaries o “wee beasties” in fresh water (initial study of microbiology) our compound microscopes are called _______light____________ microscopes parts of microscope (diagram sheet) and their functions o lenses: 1. eyepiece lens: 10x 2. objective lenses: very low: 5x (total= _50x___) low: 10x (total= _100x___) high: 43x (total= __430x__) oil: 97x (total= __970x__) light microscopes limited to 2000x why? __wavelength of light_____________________________________________________________ resolution drops with increase in magnification resolution= __detail of image___________________________________________________________ unit of measure = μ micron (SI unit of length measurement) = 0.000001m 1 millimeter = 1,000 microns 2mm=2000 μm; 4.5mm=4500 μm; 65 μm= 0.065mm TEM’s: Transmission Electron Microscopes use electrons in place of ____light__________ focus with magnets electron beam passes through thin section of object (therefore not alive) magnifies 200,000x: photo enlarged 5x: total= __1,000,000x_______ (no resolution problems) SEM’s: Scanning Electron Microscopes shows surface of object in 3D allows very ___high_______ magnification Other Tools of the Biologist Ultra Centrifuge ex. separating blood or urine samples ex. separating cell components based on __density___________ Chromatography paper column Electrophoresis gel Computers data management 15 Name: ____________________________________ Microscope Label the parts of the microscope. Per. ________ 7. 1. coarse adjustment knob 2. fine adjustment knob ocular/ eyepiece 8. body tube 9. arm 3. stage clips 4. 10. low power objective 11. high power objective 12. 5. (inclination joint) 13. 6. base 16 nosepiece 14. stage diaphragm light source Regents Questions: 1. The four wells represented in the diagram were each injected with fragments that were prepared from DNA samples using identical techniques. 2. A laboratory technique is illustrated in the diagram below. This laboratory procedure is known as (1) cloning (2) gel electrophoresis (3) chromatography (4) use of a dichotomous key This technique is used to (1) determine volume (2) separate molecules in a mixture (3) measure length (4) analyze data from an experiment 3. DNA electrophoresis is used to study evolutionary relationships of species. The diagram below shows the results of DNA electrophoresis for four different animal species. Which species has the most DNA in common with species A? (1) X and Y, only (3) Z, only (2) Y, only (4) X, Y, and Z 17 Microscope Questions: 1. A wet-mount slide preparation of a thread viewed in the low-power field (100x) of a compound light microscope is shown in diagram A below. Diagram B shows the field of view as it appeared when the objective was switched to high power. Which statement best explains why the thread is not visible in diagram B? ____The specimen was not centered. _________________________________________________________ 2. The diagram below shows an amoeba moving out of the high-power field of view of a compound microscope in the direction indicated by the arrow. What should be done to center the amoeba in the field of view and focus it sharply? __Move the slide to the right __________________________________________________________________ __Focus using the fine adjustment._____________________________________________________________ 3. The size of the image of a cell being examined with a microscope is determined by (a) light source and fine adjustment (c) objectives and ocular (b) stage and stage clips (d) diaphragm and coarse adjustment 4. The diagram below represents a hydra as viewed with a compound microscope. If the hydra moves toward the right of the slide preparation, which diagram best represents what will be observed through the microscope? 18 5. The diagram below represents two cells next to a metric measuring device under the low power objective of a compound light microscope. What is the approximate length of a nucleus of one of these cells? (a) 100m (b) 500 m (c) 1,000m (d) 1,500 m Use the diagram below to answer questions 6 and 7. 6. What is the lowest possible magnification that can be obtained using the microscope shown below? _____20 x 10 = 200x___________________________________________________________ 7. A student observes 12 onion epidermal cells along the diameter of the low power field. How many of these cells would the student observe along the diameter of the high power field? Explain. ____low power: 200x; high power: 800x… high power magnifies images 4x than low power. ____12 / 4 = 3 cells ___________________________________________________________ ______________________________________________________________________________ 8. To locate a specimen on a prepared slide with a compound microscope, why should a student begin with the low power objective instead of the high power objective? ___greater field of view. ________________________________________________________ 19 9. Explain how the light intensity in the high power field of view of a compound microscope may be increased? __use the diaphragm_________________________________________________________ 10. Each division of the metric ruler shown in the diagram below equals 1 millimeter. The diameter of the field of vision is approximately (a) 2,800m (b) 3,700m (c) 4,400m (d) 4,700m 11. The diagram below represents cells in a microscopic field of view with a diameter of 1.5 mm. What is the approximate length of a single cell? Show calculations. __1.5/3 = 0.5mm OR 1.5mm = 1500 μm… 1500/3 = 500 μm __________________________ Base your answers to questions 12 and 13 on the diagram of the single celled organism observed by a student using the low power objective of a microscope. 12. How should the student should move the slide on the stage to center the organism in the field? (a) away from herself and to her right (c) toward herself and to her right (b) away from herself and to her left (d) toward herself and to her left 13. As the student observes the organism under the high power objective, the organism swims out of focus. To bring it back into focus, the student should (a) open the diaphragm (c) turn the ocular (b) turn the fine adjustment (d) adjust the light source 20 14. The coarse adjustment of a compound light microscope should be used to (a) focus the image of a specimen under the low power objective (b) focus the image of a specimen under the high power objective (c) increase the light intensity passing through a specimen (d) measure the diameter of the high power field 15. A compound light microscope has a 5x ocular and a 10x low power objective. Calculate the total magnification that is obtained using this low power objective. ____5 x 10 = 50x______________________________________________________________ 16. The ocular of a compound light microscope has a magnification of 10x, and the low power objective and high power objective lenses have magnifications of 10x and 30x, respectively. If the diameter of the low power field measures 1,500 micrometers, the diameter of the high power field will measure (a) 100m (b) 300m (c) 500m (d) 4,500m 17. During the preparation of a wet mount, a student dropped a plastic cover slip directly on top of the drop of water containing the specimen. This slide preparation technique most probably (a) caused the cover slip to shatter (b) crushed the specimen (c) trapped air bubbles under the cover slip (d) scratched the surface of the slide 18. The threads of different colors, one blue, one green and one red, were placed on a slide so they crossed over one another. The red thread was placed on the bottom, the green one in the middle and the blue on top. The area where the threads crossed was then viewed using the high power objective. Only the blue thread was clearly visible. Describe one adjustment that could be made to obtain a clear image of only the red thread in this field of view. ___Use the fine adjustment knob._________________________________________________________________________ Base your answers to 19 and 20 on the diagram below. 19. After part 3 is turned, which part must often be adjusted? _____2_____________ 20. Which part must be adjusted to enable the viewer to continually observe a paramecium swimming at changing levels in a wet mount under high power?________ 2_____________ 21 Unit 1 Crossword Use the terms from Unit 1 to complete the puzzle. Across 1. study of the classification of living things Down 2. lenses attached to revolving nosepiece 4. 3. information collected during an experiment 6. type of reproduction employed by bacteria and protozoans 5. the unit of structure and function in this adjustment is used on low power only living things 8. ___ power is the shortest objective lens on the microscope 7. 10. changes in organisms through time 11. a _____cellular organism has many cells performing different functions 12. number of parents required for sexual reproduction 17. an organism having a single cell that carries out all life functions 19. unit of meausre; same as micrometer 21. all chemical processes that maintain life in an organism 22. provides the energy for life on earth detail of visible image under microscope 9. another name for the eypiece lens 13. this adjustment is used under high power 14. controls amount of light passing through specimen 15. a green ___ is an example of an autotroph 16. a possible solution to a problem; "educated guess" 24. number of parents necessary for asexual 18. discovered "wee beasties" reproduction 20. type of microscope that has more than one 25. type of electron microscope that lens requires thin sections 23. holds slide on stage 26. electron microscope that shows 3D image 28. longest objective lense with highest of surface magnification 27. deoxyribonucleic acid; part of chromosomes 28. the ability of an organism to maintain balance or "steady state" 22 Name: __look at the key on teacher website___ Per. _____ Unit 1 Crossword 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 23 24 21 22 25 26 27 28 23