1.
Standard Operating Procedure for the activity: Myosin Heavy Chain Procedure
2. Chemical(s) Used
Acrylamide, electrophoresis grade
3. Hazard Class
toxic
Bis-acrylamide
Tris (Hydroxymethylaminomethane)
SDS
toxic
irritant
toxic, harmful
sternutator
flammable, corrosive,
harmful if inhaled/
swallowed
oxidizing, corrosive,
harmful if swallowed
TEMED
Ammonium persulfate
4. Health Hazards
irritant to eyes, skin; may cause
cancer, genetic damage, infertility,
sensitization; nerves, kidneys
same as above
eyes, respiratory system, skin
irritant to eyes, respiratory system,
skin; sensitization; targets lungs
may cause burns; destructive to
eyes, skin, respiratory system,
mucous membranes
GI disturbances; destructive to
eyes, skin, respiratory system,
mucous membranes
eyes, skin, targets kidneys
eyes, skin, respiratory system,
mucous membranes
Glycerol
Glycine
irritant
harmful, irritant
Mercapthoethanol
Bromophenol blue
EDTA
avoid contact, inhalation, ingestion
no known hazards
5. Personal Protective Equipment required for this activity:
safety goggles, lab coat, rubber boots, nitrile gloves
6. Engineering Controls required for this activity:
fume hood, proper storage facilities
7. Other precautions required for this activity:
do not expose TEMED to vapor, follow proper handling and decontamination procedures