GENTAUR BVBA Product Datasheet Avenue de l’Armée 68/B4 B-1040 Brussels BELGIUM Phone: +32 2 732 56 88 Fax: +32 2 732 44 14 Email: info@gentaur.com http://www.gentaur.com Product: Anti-HIS1 (N-150) (Human), Affinity-Purified Polyclonal IgY Antibodies Catalog Number: A22168A Lot Number: 03BA0005-0407201 Quantity/Volume: 0.1mg/0.1 ml Target Description: HMBA-inducible; likely ortholog of mouse cardiac lineage protein 1 Target Information: Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. The function of its protein product is not yet known. Protein GI Number: 5453682 Protein Accession #: NP_006451.1 Immunogen Information: aa 1-150 Related Reagents: 1. Goat anti-IgY Fc polyclonal antibodies 2. Rabbit anti-IgY polyclonal antibodies 3. Mouse anti-IgY Fc monoclonal antibody Background References: Huang, F.; Wagner, M.; Siddiqui, M. A. Q. : Structure, expression, and functional characterization of the mouse CLP-1 gene. Gene 292: 245-259, 2002. Western Blot Test: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Species Reactivity: Human Produced From: Chicken Buffer: Phosphate-Buffered Saline. No preservatives added Storage Instruction: 4ºC for short term (weeks) and -20ºC for long term. Avoid frequent freeze and thaw. Recommended Application: Western blotting, tissue or cell immunostaining. Recommended starting dilution for Western blot analysis is 1:500, for tissue or cell staining is 1:200. Optimal working dilutions must be determined by the end user. Important Notes: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Recommended Control: Testing antigen control, Pooled pre-immune background control. __________________________________________________________________________________ For research use only, not for diagnostic or therapeutic use.