Supplementary Table 1. Primary and secondary antibodies used for retinal immunohistochemistry.
Target antigen
Primary antibody
details and working
dilution
Manufacturer
Targeted cell
population
Secondary antibody and
concentration (all from
Invitrogen Corp, Carlsbad, CA)
Cone arrestin
(LUMif)1
Rabbit polyclonal
1:5000
Kind gift of Cheryl M.
Craft, University of
Southern California
Cone
photoreceptors2
Alexafluor 546 goat anti-rabbit
1:500
Green fluorescent
protein
Rabbit FITC
conjugated, 1:1000
Invitrogen Corp,
Carlsbad, CA
GFP transduced
cells2
N/A
L/M-opsin
Rabbit polyclonal
1:500
Millipore, Billerica, MA
L/M-cone outer
segments3
Alexafluor 546 goat anti-rabbit
1:500
S-opsin
Rabbit polyclonal
1:500
Millipore, Billerica, MA
S-cone outer
segments2
Alexafluor 546 goat anti-rabbit
1:500
Protein kinase C
alpha
Mouse monoclonal
1:500
BD Biosciences, San
Diego, CA
Rod bipolar
cells2
Alexafluor 546 goat antimouse 1:500
Neuronal Nuclei
Mouse monoclonal
1:2000
Millipore, Billerica, MA
Ganglion and
Amacrine cells4
Alexafluor 546 goat antimouse 1:500
Laminin5
Rabbit polyclonal
Dako, Carpinteria, CA
Inner limiting
membrane6
N/A
1.
Li A, Zhu X, Brown B, Craft CM. Gene expression networks underlying retinoic acid-induced
differentiation of human retinoblastoma cells. Invest Ophthalmol Vis Sci 2003; 44(3): 996-1007.
2.
Mowat FM, Gornik KR, Dinculescu A, Boye SL, Hauswirth WW, Petersen-Jones SM et al. Tyrosine
capsid-mutant AAV vectors for gene delivery to the canine retina from a subretinal or
intravitreal approach. Gene Ther 2014; 21(1): 96-105.
3.
Mowat FM, Breuwer AR, Bartoe JT, Annear MJ, Zhang Z, Smith AJ et al. RPE65 gene therapy
slows cone loss in Rpe65-deficient dogs. Gene Ther 2012; 20: 545-555.
4.
Johansson UE, Eftekhari S, Warfvinge K. A battery of cell- and structure-specific markers for the
adult porcine retina. J Histochem Cytochem 2010; 58(4): 377-89.
5.
Zarfoss MK, Klauss G, Newkirk K, Kiupel M, Jones Y, Colitz CM et al. Uveal spindle cell tumor of
blue-eyed dogs: an immunohistochemical study. Vet Pathol 2007; 44(3): 276-84.
6.
Dalkara D, Kolstad KD, Caporale N, Visel M, Klimczak RR, Schaffer DV et al. Inner limiting
membrane barriers to AAV-mediated retinal transduction from the vitreous. Mol Ther 2009;
17(12): 2096-102.