GENTAUR BVBA
Product Datasheet
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B-1040 Brussels
BELGIUM
Phone: +32 2 732 56 88
Fax: +32 2 732 44 14
Email: info@gentaur.com
http://www.gentaur.com
Product: Anti-CCNE2 (Human), Affinity-Purified Polyclonal IgY Antibodies
Catalog Number: A22404F
Lot Number: 02BA0742-031223
Quantity/Volume: 0.1mg/0.1ml
Target Description: Cyclin E2 isoform 3
Target Information: This protein belongs to the
highly conserved cyclin family, whose members are
characterized by a dramatic periodicity in protein
abundance through the cell cycle. Cyclins function as
regulators of CDK kinases. Different cyclins exhibit
distinct expression and degradation patterns which
contribute to the temporal coordination of each mitotic
event. This cyclin forms a complex with and functions
as a regulatory subunit of CDK2. This cyclin has been
shown to specifically interact with CIP/KIP family of
CDK inhibitors, and plays a role in cell cycle G1/S
transition. The expression of it’s gene peaks at the
G1-S phase and exhibits a pattern of tissue specificity
distinct from that of cyclin E1. A significantly increased
expression level of this gene was observed in tumorderived cells. Three alternatively spliced transcript
variants, which encode distinct isoforms, have been
reported.
Recommended Control: Testing antigen control,
Pooled pre-immune background control.
Related Reagents:
1. Goat anti-IgY Fc polyclonal antibodies
2. Rabbit anti-IgY polyclonal antibodies
3. Mouse anti-IgY Fc monoclonal antibody
Background References: Gudas, J. M.; Payton, M.;
Thukral, S.; Chen, E.; Bass, M.; Robinson, M. O.;
Coats, S. : Cyclin E2, a novel G1 cyclin that binds
Cdk2 and is aberrantly expressed in human cancers.
Molec. Cell. Biol. 19: 612-622, 1999.
Western Blot Test:
E coli-derived fusion protein as test antigen. Affinitypurified IgY dilution: 1:2000, Goat anti-IgY-HRP
dilution: 1:1000. Colorimetric method for signal
development.
Protein GI Number: 17318567
Protein Accession Number: NP_004693.2
Immunogen Information: aa 1-296
Species Reactivity: Human, mouse, rat.
Produced From: Chicken
Buffer: Phosphate-Buffered Saline
Storage Instruction: 4ºC for short term (weeks) and
-20ºC for long term. Avoid frequent freeze/thaw.
Important Note: During shipment, small volumes of
product will occasionally become entrapped in the
seal of the product vial. For products with volumes of
200 µL or less, we recommend gently tapping the vial
on a hard surface or briefly centrifuging the vial in a
tabletop centrifuge to dislodge any liquid in the
container’s cap.
Recommended Application: Western blotting, tissue
or cell immunostaining. Recommended starting
dilution for Western blot analysis is 1:500, for tissue or
cell staining is 1:200. Optimal working dilutions must
be determined by the end user.
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For research use only; not for diagnostic or therapeutic use.