UC IRVINE INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE
APPENDIX B
CREATE AND MAINTAIN A BREEDING COLONY
Lead Researcher Name: Type here
INDIVIDUAL RESPONSIBLE FOR COLONY MAINTENANCE
NAME: Type here
HOME DEPT: Type here
WORK PHONE #: Type here
EMERGENCY PHONE (after hours) #: Type here
UCI.EDU E-MAIL ADDRESS: Type here
Describe this individual’s training and experience as it relates colony maintenance and breeding of
animals:
Type here
OTHER RESEARCH PERSONNEL INVOLVED IN MAINTAINING THE COLONY
Name:
Name:
Name:
Type here
Type here
Type here
Email Address:
Email Address:
Email Address:
Type here
Type here
Type here
Describe their training and experience as it relates colony maintenance and breeding of animals:
Type here
A. Please provide the following information about the breeding colony after you have
reviewed the IACUC Policy on the Maintenance of Breeding Colonies:
1. Provide a scientific justification for establishing and maintaining a breeding colony of animals at
UCI:
NOTE: Cost savings alone is not a valid justification for maintaining a breeding colony.
Type here
2. Describe any variations from standard ULAR-provided husbandry, care or housing required by
the breeding colony:
NOTE: If you intend to provide your own husbandry and care for the animals, please complete
Appendix I and submit it with your application.
Type here
Appendix B
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3. Describe the breeding scheme that will be used (monogamous pairs, harem, etc.):
Type here
4. Describe how overcrowding of breeding cages will be avoided, particularly when harem-breeding
schemes are used (e.g., females are separated when visibly pregnant):
Type here
5. Indicate the age at which animals are weaned. A scientific justification is required for animals
that remain with the dam longer than 21 days (see Policy referenced above):
Type here
6. Indicate with an “X” in the bracket [
] how the animals will be genotyped:
Sample type:
[ ] Tail clip
[ ] Blood sample--Describe the collection procedure: Type here
[ ] Other (describe): Type here
Age of animals when sample is taken:
[ ] 0-14 days
[ ] 14 days – 20 days (pre-weaning)
[ ] 21 days (weaning)
[ ] 22 days and older (adult post-weaning)
Method of anesthesia (required for animals genotyped at or above weaning age): Type here
Method of identification:
[ ] PCR
[ ] Southern
[ ] Coat color
[ ] Other (describe): Type here
7. Describe how the health and maintenance of the colony is managed and recorded. Include a
brief description of the record-keeping procedures that will be used to track pedigree, breeding
performance, number of animals produced by the colony, etc.:
Type here
8. Describe any known or expected phenotypes (behavioral, anatomical or physiological) of animals
produced in the colony. If the phenotype may adversely affect the health and welfare of the
animals, describe how animals will be monitored and treated for pain, distress and discomfort:
Type here
9. Indicate with an “X” in the bracket [
identified:
] how animals in the breeding colony will be individually
Appendix B
2
[
[
[
[
[
[
] Ear notch
] Ear tag
] Tattoo
] Microchip implant
] Cage card only
] Other (describe): Type here
NOTE: Toe clipping as a method of identification requires a scientific justification; less invasive
methods are strongly recommended.
10. Indicate whether breeder animals will receive any experimental or therapeutic drugs, ovulation
agents or in-utero therapies or therapeutics. List the agent, dosage and frequency of
administration if applicable:
Type here
11. Indicate how excess or unused animals produced in the colony (i.e.: animals with the wrong
genotype, retired breeders, animals not needed for the research) will be euthanized:
Type here
B. Please list in the table below the number of animals needed to establish the colony,
including founder animals, wild-type strains needed for backcrossing, etc.
NOTE: If this is a renewal application for an existing colony, please list the breeder animals that
you currently have in your colony.
SPECIES AND STRAIN
EXAMPLE: Mouse Balb/c
Type here
Type here
Type here
Type here
CONDITION CODE
EXAMPLE: ADULT-S
Type here
Type here
Type here
Type here
TOTAL # OF ANIMALS
EXAMPLE: 100
Type here
Type here
Type here
Type here
C. Please estimate the approximate number of animals that will be produced in the colony and
retained as replacement breeding animals during the three-year protocol period.
SPECIES AND STRAIN
Type here
CONDITION CODE
Type here
TOTAL # OF ANIMALS
Type here
D. Please estimate the approximate number of animals that will be produced in the colony but
not used for research purposes or retained as breeders (e.g., culled littermates, wrong
genotype). Provide a brief explanation of why they will not be used, and describe what will
be done to minimize this number.
Approximate number not used:
Type here
Explanation of why they will not be used:
Type here
Appendix B
3
What will be done to minimize this number:
Type here
NOTES:
Investigators are responsible for the reporting of all animals that are produced in UCI breeding
colonies. The following animals must be reported via the web:
All offspring from breeding colonies must be reported, including:
 Pups culled from litters
 Animals retained in the colony to be used as breeders
 Animals used for research purposes (reported in the appropriate USDA pain category)
In addition, the live-born offspring of animals that are purchased as Pregnant or Dams-With-Litter
must be reported via the web. See Reporting Animal Numbers.
Appendix B
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