Flow Cytometry/Confocal Microscopy Facility
(For more information, see the ECU Cytometry Core web page)
Location:
4W37 Brody Medical Sciences Building
Contact:
Douglas A. Weidner, PhD
Office: (252) 744-3245
Lab: (252) 744-2799
weidnerd@ecu.edu
Purpose
To provide multiple data acquisition and analysis capabilities in flow cytometry (including cell sorting), confocal
microscopy, and laser scanning cytometry. To train faculty, staff and students in operation of these instruments
and assist in computer data analysis and interpretation.
Capabilities
Flow Cytometry (partial list of applications)
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Immunophenotyping
Cell cycle analysis
Up to eleven-color fluorescence analysis
Clonal sorting and deposition
Calcium and magnesium intracellular flux
Membrane potential studies
Confocal Microscopy
Localization and co-localization of biomolecules in tissues or cells and 3-D reconstruction of fluorescently
labeled cells or tissues
Laser Scanning Cytometry
Similar capabilities as flow cytometry (except sorting) on a slide or multi-well plate format, with up to four-color
fluorescence analysis
Fees for Services
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Flow Cytometry: $50/ hour
Flow Sorting: $50/ hour, plus $25 set-up fee
Confocal Microscopy: $40/ hour
Laser Scanning Cytometry (iCys): $40/ hour
Instrumentation
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Polychromatic Flow Cytometry System (Four Laser, Eleven Color)-Becton Dickinson LSR II (See
www.bdbiosciences.com/instruments/lsr/products/lsrii.jsp for more information)
Three bench-top flow analyzers (three color) - Becton Dickinson FACScan
Cell sorter - Becton Dickinson FACSVantage SE with high-speed sorting capability (Turbo-sort), with three
lasers, extending from UV to far red wavelengths
Computer workstations with USB thumb-drive and CD-RW data storage
Confocal Microscope- Zeiss LSM 510 with 3 lasers and 3 fluorescence detectors
Laser Scanning Cytometer- CompuCyte iCys (location- Brody 7S-19)
(For more information, see www.compucyte.com/icys.htm)
Agilent 2100 BioAnalyzer (location- Brody 3E-94)
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