SOP Number: CP034.00
Title: Preparation of Blood, Tissue, and Urine Sample
Containers
Revision No: Replaces:
00
N/A
Author: Tatiana Zanganeh
I
Date in effect: Page:
7/8/2010
Page 1 of 4
MAL Director: Dr. Peggy Borum
FSHN Chair: Dr. Neil Shay
PURPOSE
Tissue and blood sample containers are used to transport and store samples so they can be used
for analysis and/or assays.
II
SCOPE
This procedure outlines the preparation of containers for storing tissue and blood samples.
During non-survival surgery, organs may be collected for analysis. It is important to correctly
label these containers so they can be properly organized and used later. Assigning each piglet a
‘piglet identification number’ is an easy way to keep track of samples.
III
RESPONSIBILITIES
It is the responsibility of Metabolic Assessment Laboratory personnel to follow this procedure.
It is the responsibility of supervisory personnel to ensure compliance with this procedure and to
train employees and students responsible for performing this procedure. Students will report
accidents to the principal investigators immediately.
IV
REFERENCES
N/A
V
REAGENTS AND MATERIALS
V.A.
V.B.
V.C.
V.D.
V.E.
V.F.
V.G.
V.H.
V.I.
V.J.
VI
Plastic, white capped 20mL scintillation vials.
Vacutainer purple-topped blood collection tubes.
Plastic test tubes with removable plastic tops.
Large plastic weigh boats.
Small plastic weigh boats.
Printer paper.
Clear tape.
Fine-tipped black marker.
Scintillation vial tray.
Scissors.
EQUIPMENT
N/A
VII
SAFETY PRECAUTIONS
 Uncontrolled Copy
Document1
CONTROLLED DOCUMENT-DO NOT DUPLICATE
 Controlled Copy No._______
SOP Number: CP034.00
Title: Preparation of Blood, Tissue, and Urine Sample
Containers
Revision No:
00
Replaces:
N/A
Date in effect:
7/8/2010
Page:
2 of 4
VII.A. Members of the MAL have been trained extensively in the procedures described in this
SOP.
VIII
DEFINITIONS
VIII.A. Standard Operating Procedure (SOP) – Standard Operating Procedure is a document that
provides instructions for completing a specific task in the lab.
VIII.B. Metabolic Assessment Laboratory (MAL) – The Metabolic Assessment Laboratory is the
laboratory that will use this SOP.
VIII.C. Piglet Identification (ID)—The Piglet ID is a unique, alphabetical and numerical label
that identifies the piglet and the samples taken from that piglet.
IX
PROCEDURE
IX.A.
IX.B.
IX.C.
Preparation of the tissue collection weigh boats.
IX.A.1. Refer to Attachment I for number of weigh boats needed.
IX.A.2. Label the outer edge of the plastic weigh boat in the following manner with a
fine tip black permanent marker:
IX.A.2.a
Piglet ID.
IX.A.2.b
Name of organ.
Preparation of the tissue collection vials.
IX.B.1. Refer to Attachment I for number of vial labels needed.
IX.B.2. Using a fine tip black marker, label the top of the plastic vial with the following:
IX.B.2.a.
Piglet ID.
IX.B.2.b.
Name of organ.
IX.B.3. Tape a paper label to the side of the vial making sure to cover the paper label
and encircle the vial completely with tape to ensure a water tight seal with the
following information:
IX.B.3.a. Piglet ID.
IX.B.3.b. Name of organ.
IX.B.3.c. Date of non-survival surgery.
IX.B.4. Be sure to evenly center the label on the vial and wrap the tape around
completely.
Preparation of the blood collection Vacutainer tubes.
IX.C.1. Tape a paper label to the side of the Vacutainer tube perpendicular to the length
of the Vacutainer tube, using the same technique as above with the following
information:
IX.C.1.a. Piglet ID.
IX.C.1.b. “Central Blood” or “Portal Blood”.
IX.C.1.c. Date of non-survival surgery.
IX.C.2. Repeat the procedure with the correct number of Central Blood and Portal
Blood Vacutainer tubes.
 Uncontrolled Copy
Document1
CONTROLLED DOCUMENT-DO NOT DUPLICATE
 Controlled Copy No._______
SOP Number: CP034.00
Title: Preparation of Blood, Tissue, and Urine Sample
Containers
Revision No:
00
IX.D.
IX.E.
Replaces:
N/A
Page:
3 of 4
Preparation of test tubes for blood analysis.
IX.D.1. Refer to the following information to tape paper labels to plastic test tubes
perpendicular to the length of the test tube using the technique mentioned
above.
IX.D.1.a. Piglet ID.
IX.D.1.b. Name of sample.
IX.D.1.c. Date of non-survival surgery.
IX.D.2. Repeat the procedure with the following number of blood analysis tubes per
piglet:
IX.D.2.a.
14 Washed RBC.
IX.D.2.b. 40 Plasma.
IX.D.2.c.
1 Blood for Hgb.
IX.D.2.d. 1 Portal for Hgb.
IX.D.2.e.
3 Portal Plasma.
IX.D.2.f.
3 Portal Washed RBC.
Preparation of the 20mL scintillation vial for urine collection.
IX.E.1. Label 20 mL scintillation vial caps with a fine tip permanent marker the
following information:
IX.E.1.a. Piglet ID.
IX.E.1.b. Urine.
IX.E.1.c. Date of urine sample.
IX.E.1.d. Time of hourly urine draw.
IX.E.2. Tape a paper label using the technique mentioned above to the side of the
plastic vial as follows:
IX.E.2.a. Piglet ID.
IX.E.2.b. Urine.
IX.E.2.c. Date of urine sample.
IX.E.2.d. Time of hourly urine draw.
IX.E.3. A separate vial needs to be prepared for every two samples of urine drawn on
the half hour (one vial every hour).
 Uncontrolled Copy
Document1
Date in effect:
7/8/2010
CONTROLLED DOCUMENT-DO NOT DUPLICATE
 Controlled Copy No._______
SOP Number: CP034.00
Title: Preparation of Blood, Tissue, and Urine Sample
Containers
Revision No:
00
X
Replaces:
N/A
Date in effect:
7/8/2010
Page:
4 of 4
ATTACHMENTS
X.A. For a list of materials and their locations, refer to SOP# CG098.00.
X.B. Number of Vials and Weigh Boats.
Organ
Adrenals
Bile
Heart
Kidneys
Liver
Lung
Muscle
Spleen
Stomach
Stomach contents
Top 1/3rd of SI
Top 1/3rd of SI contents
Middle 1/3rd of SI
Middle 1/3rd of SI contents
Bottom 1/3rd of SI
Bottom 1/3rd of SI contents
Large Intestine
Large Intestine Contents
Left Cerebrum
Left Cerebellum
Right Caudate Nucleus
Right Cerebellum
Right Frontal Lobe
Right Hippocampus
Right Hypothalamus
Right Lateral Temporal Lobe
Right Medulla
Right Occipital Lobe
Right Parietal Lobe
Right Pons
Right Thalamus
 Uncontrolled Copy
Document1
Number of Vials (49)
1
1
3
2
4
3
3
1
2
3
2
1
2
1
2
1
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Size of Weighboat
Small
Small
Small
Large
Large
Large
Large
Small
Small
n/a
Large
n/a
Large
n/a
Large
n/a
Large
n/a
Small
Small
Small
Small
Small
Small
Small
Small
Small
Small
Small
Small
Small
CONTROLLED DOCUMENT-DO NOT DUPLICATE
 Controlled Copy No._______