SOP Number: CP053.00
Title: Preparing PNICU for Experiment 1
Revision No:
00
Replaces:
N/A
Date in effect:
7/15/2010
Author: Tatiana Zanganeh
I
Page:
Page 1 of 4
MAL Director: Dr. Peggy Borum
FSHN Chair: Dr. Neil Shay
PURPOSE
The purpose of PNICU preparation is to clean and prepare the piglet facility before the piglet
arrives.
II
SCOPE
This procedures provides instructions on how to clean and prepare the PNICU and piglet facility
for an experiment.
III
RESPONSIBILITIES
It is the responsibility of Metabolic Assessment Laboratory personnel to follow this procedure.
It is the responsibility of supervisory personnel to ensure compliance with this procedure and to
train employees and students responsible for performing this procedure. Students will report
accidents to the principal investigators immediately.
IV
REFERENCES
N/A
V
REAGENTS AND MATERIALS
V.A. Disinfectant cleaning solution (Lysol); a mixture of 4 oz of disinfectant to 2 gallons of
water.
V.B. Shoe covers.
V.C. Trash bags.
V.D. Biohazard bags.
V.E. 1- and 2-gallon Sharps containers.
V.F. Biohazardous and radioactive labels (as needed).
V.G. Cotton cloth.
V.H. Container for cleaning solution.
V.I. Bucket for mopping solution.
VI
EQUIPMENT
VI.A. Mop with a levered squeezer.
VII
SAFETY PRECAUTIONS
VII.A. Members of the MAL have been trained extensively in the procedures described in this
SOP.
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SOP Number: CP053.00
Title: Preparing PNICU for Experiment 1
Revision No:
00
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N/A
Date in effect:
7/15/2010
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VII.B. Members of the MAL have completed animal contact training in order to work in the
PNICU.
VII.C. Members have been trained on proper chemical use and handling within the fume hood
and realize that human samples are to be assayed in the biofume hood.
VIII
DEFINITIONS
VIII.A. Standard Operating Procedure (SOP) – Standard Operating Procedure is a document that
provides instructions for completing a specific task in the lab.
VIII.B. Metabolic Assessment Laboratory (MAL) – The Metabolic Assessment Laboratory is the
laboratory that will use this SOP.
VIII.C. Piglet Neonatal Intensive Care Unit (PNICU) – The PNICU is a unit where the piglet is
monitored by 24 hour care and routine check-up parameters using PNICU SOPs
conducted by the MAL.
VIII.D. Piglet Facility—The Piglet Facility is comprised of three rooms: the PNICU used for
piglet care; a surgery room used for surgical procedures; and a transition/entry room used
by staff to prepare items for care or surgery and to transfer the piglet from surgery to
care.
IX
PROCEDURE
IX.A. The Entry Room
IX.A.1. The entry room contains a hand washing sink for surgery, a general purpose sink,
and cabinet with workspace.
IX.B. Preparation of the Entry Room
IX.B.1. The entry room is the first entered of the piglet facility, and the last cleaned.
IX.B.2. Remove any entrance workspace surfaces and cabinets from the entry room
before cleaning.
IX.B.3. Clean the ceiling, light reflector covers, walls, doors and door jams, and sinks
with a disinfectant cleanser solution and a cotton cloth.
IX.B.4. Clean the floor with a disinfectant cleanser solution. Use the mop with a levered
squeezer and bucket to clean the floor. This mop is exclusively reserved for
cleaning the piglet facility. Shoe covers must be worn from this point onward.
IX.B.5. After the room is dry, clean any main entrance workspace and cabinet with a
disinfectant cleanser solution and a cotton cloth.
IX.B.6. Place the following items by each sink:
IX.B.6.a. Hand brush
IX.B.6.b. Antiseptic hand cleanser
IX.B.6.c. 70% Ethanol squirt bottle
IX.B.7. Place one large biohazard container (with biohazard bag) by the door to the
PNICU into which all the PNICU trash is placed. If the trash is radioactive, both
the trash bag and area will be marked radioactive.
IX.B.8. Place a biohazard sharps box on the entry room work surface area. This is the
waste container for any small, sharp materials, like needles, that are contaminated
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SOP Number: CP053.00
Title: Preparing PNICU for Experiment 1
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00
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Date in effect:
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with any biohazardous material.
IX.B.9. A mop with a levered squeezer, bucket, broom, and dust pan should be located to
the side of the door to the PNICU. All of these items are used only in the piglet
facility. Clean these items prior to the experiment with a disinfectant cleaner
solution and a cotton cloth.
IX.B.10. Place shoe storage shelves under each sink. These are cleaned prior to the
experiment with a disinfectant cleaner solution and a cotton cloth.
IX.B.11. On top of the workspace area, place the following prior to surgery:
IX.B.11.a. One roll of autoclaved paper towels.
IX.B.11.b. A bin for instruments that need to be cleaned.
IX.B.11.c. A sharps container.
IX.B.12. Inside the workspace cabinet place items that may be needed during the
experiments:
IX.B.12.a. Extra infusion pumps.
IX.B.12.b. Extra piglet teddies.
IX.B.12.c. Extra oxygen lines with nose funnels.
IX.B.12.d. Extra IV bag poles.
IX.B.12.e. Extra IV bag hooks.
IX.B.12.f. Extra trash bags, biohazardous bags.
IX.B.12.g. Bottle of disinfectant cleaner.
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Title: Preparing PNICU for Experiment 1
Revision No:
00
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ATTACHMENTS
X.A. For a list of materials and their locations, refer to SOP# CG098.00.
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