RAPID Wilkinson Basin Sampling Protocol
UME based trips: 2 science people at minimum, follow all the protocols.
UNH based trips: One designated UME person participates and helps where needed.
Only net sampling is required at WB-5 and WB-7, same protocols as below. Priority is
always WB-7, and if only one cast possible, then live and formalin samples are the
priority. LOPC at both stations.
Station Events: Boat knows each stations coordinates by heart.
Record all information on a “Bottle Depth sheet” as a cruise log. Print
out several, double-sided so that each station has it’s own page.
WB-5: (located on Jeffreys Ledge) CTD, 2 dual ring casts (2- 4% Form, 1 ETOH, no
live), LOPC, 5 Niskins (chl-A and TA/DIC)
WB-7: CTD, 5 Niskins (Chl-A and TA/DIC samples), 2 dual ring casts (2 Form, 1 ETOH,
1 Live), LOPC
Protocols:
LOPC: one cast each station at 40 m/min
Dual Ring Net:
Two Casts of 5m from bottom @ 40m/min.
Record start and end flowmeter numbers and depth net went to.
Record which cast was preserved as what.
Rinse nets designated for preserved samples, but not live net
Put labels inside jar and out
Be sure to fill out labels with cast and net number and be sure preservative label is
correct to match which cast goes with what.
1. Cast 1:
a. One net with COVERED (black tape over holes) live codend to be
divided up among live jugs. Rinse jugs first with hose water. Fill jugs
with left over Niskin bottle water. No need to use ALL of the live codend contents- just add enough for about 100 animals per liter.
b. One net preserved in 4% Formalin/seawater (rinse sample down with
seawater; plop sample into jar with concentrated formalin; top off with
seawater to shoulder of jar)
2. Cast 2:
a. Switch out covered live codend for a filtering type (not duct taped)
b. One net preserved in 95% ETOH:
i. Use the 150um mesh sieve for ETOH
ii. Condense all sample to one edge of sieve with seawater
iii. Use ETOH filled squirt bottle to move sample from sieve to jar,
top off with ETOH.
c. One net for 4% Formalin/seawater
3. At end of each cruise, check that flowmeters count revolutions and check nets
for rips and tears. Replace/repair where needed
CTD/Niskins:
1.
2.
3.
4.
5.
Instrument cast to within 5 meters off bottom
Remove PAR cap (if present)
Attach big weight to bottom of SeaBird cage
Doesn’t have to be slower than 10m/min
R/V Gulf Challenger crew likes to do Niskin Bottles on same wire as CTD.
Must fill out Bottle Depth sheet included with this protocol (Dropbox/Wilki
RAPID cruises/Meter_Wheel_Cal.xls). Best to download file to laptop to be
done in wheelhouse by science member via computer. Trust me, you’ll
appreciate that.
6. Use the Bottle Depth to calculate for the crew running the winch the depths to
be stopped at for attaching Niskins to the wire.
7. Depths for bottle placement: 2m, 10, 20, 40, 120m and deep (just above CTD)
where applicable for a total of 6 Niskins.
Water Filtering (to be done after station):
1. Chl-A: ONLY ON UME TRIPS:
a. Label centrifuge tubes with: Date, Rep, Filter type (GF, 5um 20um) and
Depth
b. 2m= 2 replicates GF/F; 1 TA/DIC bottle
c. 10m= 2 reps GF/F; 2 rep 5m, 2 reps 20m
d. 20m= 2 reps GF/F
e. 40m= 2 reps GF/F
f. 120m= 2 TA/DIC bottles
g. Deep (just above CTD, record depth)= 2 TA/DIC bottles
VERY important to follow protocols for TA/DIC water collection. USE
GLOVES when handling the mercury preservative.
h. Wrap centrifuge tubes in foil and put under ice. Put in -80C at GMRI as
soon as possible after trip.
TA/DIC: we take care of on UME trips/ on UNH trips they do it:
Follow protocols UNH left us. Grab the kit and bottles from their shed. Clean bottles
should be in a dark blue tote box just inside the UNH groups’ storage bay door. Bryan,
the Captain, will open the storage bay for you. When done with the trip, full bottles and
kit go back in shed and email goes out to Chris Hunt.
2 samples per 2m, 120m and deep- no bubbles in water hose and small bubble to allow
expansion during freezing.
Equipment List and Location of Items:
Item
CTD
Niskins
Niskin hanging rack
Weights
Dual ring
Cod ends
Filtering Manifold
Filter bottle (for vacuum)
Filter pump
150 um Sieve/squirt
bottles
TC/DIC kit and 3 bottles
ETOH carboy
Buffered Form primed
jars
Empty ETOH jars
Live jugs
Green cooler
LOPC
HUGE round weight
GF/F, 5um and 20um
filters, tweezers, foil
Large centrifuge tubes
ICE
Meter wheel sheet
Location of storage
Subject to change
UNH storage @ Newcastle
UNH storage
UNH (in big basket of bottles
and or black plastic tackle box)
UNH
UNH
UNH
UNH
UNH
UNH
# Needed
1
5
1
6
UNH
GMRI
GMRI
1
1
4
GMRI
GMRI
GMRI
GMRI
UNH
GMRI
2
7-8
1
1
1
1 box each
GMRI
GMRI/gas station
On computer or printed out
12
2 bags
Lab Sorting of Live Sampling #’s:
Stage
Genetics (-80)
RNA/DNA (-80)
(individual)
(individual)
CV
30
30
Female
30
30
1
1 covered, 2 regular
1
1
1
1
Fatty Acid (DEWAR)
(3 indiv per tube)
10 vials = 30 animals
10= 30 animals
DATE:
GC WILKI CRUISES
STATION:
INDICATE WHETHER
JOINT UNH TRIP OR UME ONLY
LAT:
LONG:
TIME:
WATER DEPTH:
Actual Desired Depths:
Sample Desired Depths:
Bottle # 1:
2m
Bottle # 1:
m
(U)
Bottle # 2:
m
(V)
Bottle # 3:
m
(W)
Bottle # 4:
m
(X)
Bottle # 5:
m
(Y)
CTD:
m
(Z)
Bottom:
m
(U)
Bottle # 2:
(V)
Bottle # 3:
(W)
Bottle # 4:
(X)
Bottle # 5:
(Y)
CTD:
(Z)
Bottom:
RING NET FLOWMETER:
RECORD EACH RING NET CAST DEPTH!!
RECORD MESSENGER DROPPED TIME!
Meter Wheel Reading:
CAST 1
Side 1:
STARTEND-
Side 2:
START-
Attach Bottle # 5:
END-
Attach Bottle # 4:
m =(Y - X)
Attach Bottle # 3:
m =(Y - W)
Side 2:
START-
Attach Bottle # 2:
m =(Y - V)
END-
Attach Bottle # 1:
m =(Y - U)
Cast Depth:
2m
CAST 2
Side 1:
STARTEND-
Cast Depth:
Lower CTD to:
m =(Z)