OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Spherical baculovirosis
(Penaeus monodon-type baculovirus)
Institute of Zoology,
College of Life Science,
National Taiwan University,
No.1, Sec, Roosevelt Road,
Taipei 106, Taiwan,
CHINESE TAIPEI
Tel.:
(886-2) 2363-3562
Fax:
(886-2) 2363-8179
e-mail address:
website:
gracelow@ntu.edu.tw
http://shrimpwssv.lifescience.ntu.edu.tw/
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Prof/Dr Grace Chu-Fang Lo
Name(including Title and
Position) of OIE Reference
Expert:
Prof/Dr Grace Chu-Fang Lo
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
1
Spherical baculovirosis (Penaeus monodon-type baculovirus)
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
PCR
Viral genome
All known SBV genotypes
70
TEM
Viral particle
SBV collected from India
16
Production and distribution of diagnostic reagents
Type of reagent
Amount supplied nationally
(including for own use)
Amount supplied to
other countries
Control positive viral
DNA/protein
100 ampoules of 10 μl for SBV DNA and
proteins respectively
0
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
None in 2011.
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
In 2011 we prepared standard reference materials consisting of 1) SBV-infected larvae that were used as source for
SBV challenge, 2) SBV-infected tissues/larvae for positive control in protein/nucleic acid assays (for more than
1,000 reactions), 3) SBV genomic DNA fragments for positive control in PCR assay.
5.
Research and development of new procedures for diagnosis and control
Established a purification protocol for the genomic and proteomic study of SBV.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
None in 2011
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
None in 2011
8.
Provision of consultant expertise to OIE or to OIE Member Countries
None in 2011
2
Annual reports of OIE Reference Centres, 2011
Spherical baculovirosis (Penaeus monodon-type baculovirus)
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
None in 2011
10. Provision of diagnostic testing facilities to other OIE Member Countries
None in 2011
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
As Execom Chairperson (2008-2011) of the Fish Health Section (FHS) of Asian Fisheries Society (AFS), I was
involved in organizing the Eighth Symposium on “Diseases in Asian Aquaculture” (DAA8) and worked very
closely with Local Organization Committee and the International Scientific Program Committee. DAA8 was held
in Mangalore on 21-25 November, 2011. The theme of DAA 8 was “Fish Health for Food Security”. Over the
5 days, 4 thematic, 24 key note and 47 contributed papers were presented in 13 technical sessions covering
disease, diagnostics, vaccines, genomics, disease preparedness and national plans. There were also 170 poster
presentations.
12. Participation in international scientific collaborative studies
Collaboration with Prof. Jan-Ming Ho’s team at the Institute of Information Science, Academia Sinica, Chinese
Taipei to constract database of shrimp Shrimp ESTs. We summarized the sixteen major shrimp EST studies that
have been conducted to date. In addition, we analyzed the EST data downloaded from NCBI dbEST for the four
major penaeid shrimp species and constructed a database to host all of these EST data as well as our own analysis
results. This database provides the shrimp aquaculture research community with an outline of the shrimp
transcriptome as well as a tool for shrimp gene identification. All the analysis results are accessible at the
database: http://sysbio.iis.sinica.edu.tw/page/. The paper was published in Mar Biotechnol. 2011, 13:608–621.
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
None in 2011

Scientific publications in peer-reviewed journals
Leu, J. H., Chen, S. H., Wang, Y. B., Chen, Y. C., Su, S. Y., Lin, C. Y., Ho, J. M., Lo, C. F. (2011) A review of
the major penaeid shrimp EST studies and the construction of a shrimp transcriptome database based on the ESTs
from four penaeid shrimp. Mar Biotechnol 13(4), 608-21.
Huang, S. W., Lin, Y. Y., You, E. M., Liu, T. T., Shu, H. Y., Wu, K. M., Tsai, S. F., Lo, C. F., Kou, G. H., Ma, G.
C., Chen, M., Wu, D., Aoki, T., Ikuo Hirono and Yu, H. T. * (2011) Fosmid library end sequencing reveals a
rarely known genome structure of marine shrimp Penaeus monodon. BMC Genomics (12)242.

Other communications
None in 2011
_______________
Annual reports of OIE Reference Centres, 2011
3