IR Microscope
Background
The IR Microscope is used to find the composition ad bonds that can be used in the FTIR only with solids
like fibers from clothing and other fabrics. This accessory that is attached to the FTIR can also scan and
take pictures of the images of the samples.
Standard Operating Procedure
1. Make sure the instrument is on and in bypass mode.
2. Fill dewer with liquid nitrogen.
3. Insert the funnel into the top of the microscope and fill until liquid nitrogen flows out the notch
on the side.
4. Wait 20 minutes to run any samples.
5. Open Varian Resolution Pro, Collect>Rapid scan>computations.

Under computations tab
-Check to make sure these options show up:
-Compute, Ratio, Truncate, Stop (in red), absorbance to % transmittance.
6. Optics tab – Set accessory to none.
7. Place ATR crystal from white box onto the microscopes barrel.

Make sure the notches are on the left
-Slide in until you hear two clicks.
-Place the gold slide from the white box under the barrel on the stage.
-Make sure that the cracks are not located under the crystal.
8. Take a background scan.
-Background button
-Collect>rapid scan>setup
-Change the view from interferogram to processed
9. Place a known fabric sample onto the gold plate so it lines up with the crystal.
-Slowly raise the stage using the fine adjustment knowb until the lines on the computer
screen can no longer be sceen on the computer screen or until resistance is met against
the crystal.
-Make sure the known fabric sample and crystal touch completely.
-Click the four way arrow to bring the screen back.
-Hit scan.
10. After the scan, access the library by going to operations>Varian search>search.
-Spectra tab (or first tab) and search
-Record the results that match your spectra graph
-Click on its spectra graph to highlight it and it should bring up another spectra to
compare.
11. When finished, leave computer and instrument on.