Chapter 11 – Study Guide – DNA Replication and Recombination
1. Semiconservative replication
a. Other possible modes of replication that were disproven: conservative and
dispersive replication
2. Meselson-Stahl experiment
3. Taylor-Woods-Hughes experiment
4. Origin of replication
5. OriC in prokaryotes
6. Bidirectional replication
7. Replicon
8. Prokaryotic polymerases and their function (Polymerase I-V)
9. Steps that need to occur prior and during DNA replication in prokaryotes and eukaryotes
10. DnaA, DnaB, and DnaC – DNA helicases
11. Single-stranded binding proteins
12. DNA gyrase
13. DNA topoisomerases
14. Replisome
15. Primase
16. Continuous DNA synthesis
17. Leading strand
18. Discontinuous DNA synthesis
19. Lagging strand
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20. Okazaki fragments
21. DNA ligase
22. Process of DNA synthesis on lagging strand
23. Primer
24. Exonuclease activity
25. Autonomously replicating sequences (ARSs)
26. Origin Recognition Complex (ORC)
27. Types of eukaryotic DNA polymerases and their functions
28. Low processivity versus high processivity in DNA polymerases (eukaryotes)
29. Polymerase switching in eukaryotes
30. Telomeres
31. Telomere structure (repeats)
32. G-rich strand
33. C-rich strand
34. T-loop
35. G-quartets
36. Telomeres and the lagging strand problem in eukaryotic cells
37. Telomerase
38. Reverse transcription
39. Process of genetic recombination
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40. Holliday structure (chi form)
41. Heteroduplex
42. Homologous recombination
43. Gene conversion
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