MOLECULAR BIOLOGY SERVICE
SANGER SEQUENCING FACILITY
Date:………………/20__
Name: ……………………………..
Group:……….…..
Tel.:…………........
DNA (Sample Name): …….………………………………………….....................
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Primer:
 added
not added (please supply as 5 µM solution in 1xTE)
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DNA source:
 PCR fragment
 plasmid
 cosmid, BAC, YAC
DNA size: ....................basepairs total
DNA concentration: ………….ng/µl
Please note that we need about:
150 ng/rx for plasmid
10 to 50 ng/rx for PCR fragment
500 to 1000 ng/rx for cosmid, BAC, YAC
Agarose gel:
(…µl marker ….µl sample)
Place a picture with 1µl
undigested DNA sample
and size marker
Results (read length):
 500
 700
 800 and longer
If you have more than 16 reactions (e. g. 10 samples with 2 primers),
please pipette them into the plate and put an excel sample sheet into
your
folder
on
the
computer
into
“CHIPSEQ”
[\\manray\mySequence\chip_seq] or “SCRATCH” [\\manray\scratch]!
More information how to prepare the plate you can find in “Plate preparing”.