Biotechnology
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Lilli Howard
BIOL/GEN 313
Dr. Vollbrecht
04/15/14
1. Cut DNA fragments by ______________________________
2. Join DNA fragment by _______________________________
3. View DNA fragments on _____________________________
4. Locate specific DNA fragments by _____________________
5. Make copies of DNA fragments by _____________________
6. Explain how gel electrophoresis is used to separate DNA fragments of different lengths.
What are the three main components?
7. After DNA fragments have been separated by gel electrophoresis, how can they be
visualized?
8. Briefly explain how an antibiotic-resistance gene and the lacZ gene can be used to
determine which cells contain a particular plasmid.
9. Describe sticky and blunt ends. How are they made? How are they different?
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu
10. Explain how Southern Blotting is used. What are the four main components?
11. Give three important characteristics of cloning vectors.
12. What are three steps in plasmid cloning?
13. What is PCR? When do you use it? What are the three main steps? What are the four
components that you mix in the test tube?
14. Where do restriction enzymes come from?
15. DNA fragments that are 500 bp, 1000 bp and 2000 bp in length are separated by gel
electrophoresis. Which fragment will migrate farthest in the gel?
16. What are four limitations of PCR?
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu