"High Content Assay for Target Discovery using RNAi Technology"
May 11th to 15th, 2015
Institut Pasteur Korea
Pangyo Techno-valley, South Korea
This newly created course provides a learning platform for those interested or working in the fields of chemical
biology and functional genomics to interact and learn the best practices in setting up cell based assays using
automated microscopy for RNA interference (RNAi) technologies and leading to the discovery of novel gene
targets.
This course is open to a broader audience, from new comers to RNAi to seasoned research scientist familiar with
the technology, and with an interest in understanding unbiased approaches towards developing cell based assays,
using automated microscopy, performing small focused to up to genome scale screens, analyzing large RNAi
screening data sets with relevance in target discovery and cellular pathways.
There will be six main theoretical and practical short courses. The practical courses will also cover case studies on
RNAi screens.
1.
Designing & establishing cell-based assays for screening
2.
High Content Assay: instrumentation and assay development
3.
Designing & setting up assays for RNAi screening (siRNA, miRNA, & shRNA)
4.
Automated microscopy image processing and analysis
5.
RNAi screening data analysis for gene nomination, data management, and data mining
6.
RNAi data analysis in a cellular pathway context
ORGANIZER
Dr. Regis Grailhe
Technology Development Platform
Institut Pasteur Korea
South Korea
TEACHING TEAM
David Shum, Institut Pasteur Korea, South Korea
Constantin Radu, Institut Pasteur Korea, South Korea
Bhavneet Bhinder, Memorial Sloan Kettering Cancer Center, USA
Yuri Tsoy, Institut Pasteur Korea, South Korea
Regis Grailhe, Institut Pasteur Korea, South Korea
TOPICS

Designing & establishing cell-based assays for screening

High Content Assay: instrumentation and assay development

Designing & setting up assays for RNAi screening

Automated microscopy image processing and analysis

RNAi screening data analysis for gene nomination, data management, and data mining

RNAi data analysis in a cellular pathway context
Preliminary program of the course
HOW TO APPLY
People interested in participating in this course must send the application form, together with the following
documents:



short CV (max. 2 pages)
letter of support from your laboratory Head or mentor
letter of motivation
Applications should be sent to: HCA2015@ip-korea.org
The Student’s selection criteria.
. Students who plan to use High Content Screening (HCS) technology
. Students who look to implement HCS capacity at a screening center
. Students who want to optimize the RNAi screening and/or to manage and analyze data
. Students who seeking for RNAi data and pathway analysis methodologies
GENERAL INFORMATION
A maximum of 20 students will be accepted. The registration fees and meals should be offered. Travel expenses as
well as housing fees will be supported for participants belonging to the IP network.
Deadline for application is March 20th, 2015