Activities in 2009
Infectious haematopoietic necrosis
James Winton
Western Fisheries Research Center, 6505 NE 65th Street, Seattle, WA 98115 USA
Tel: 1-206-526-6587, Fax: 1-206-526-6654
E-mail: jim_winton@usgs.gov, Web site: http://biology.usgs.gov/wfrc/
Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease at your laboratory
1. Virus isolation in cell culture (diagnosis/research 42 cases).
2. Virus identification using polyclonal and monoclonal antisera (diagnosis/research 0 cases).
3. Virus identification via hybridization with biotinylated probes (diagnosis/research 0 cases).
4. Virus identification via polymerase chain reaction (diagnosis/research 109 cases)
5. Virus identification and strain typing via sequencing G or N gene regions (diagnosis/research 145 cases).
6. Determination of viral load via quantitative PCR (diagnosis/research 115 cases).
2.
Production, testing and distribution of diagnostic reagents
1. Testing and distribution of CHSE-214, EPC and other fish cell lines (6 cases).
2. Testing and distribution of polyclonal and monoclonal antisera to identify IHNV (1 cases).
3. Testing and distribution of biotinylated DNA probes to identify IHNV (0 cases).
4. Testing and distribution of reagents for polymerase chain reaction to identify IHNV (15 cases).
Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
1.
2.
3.
4.
5.
4.
Prepared revised IHN chapter for OIE Diagnostic Manual for Aquatic Animal Diseases.
Reviewed revised VHS chapter for OIE Diagnostic Manual for Aquatic Animal Diseases.
Revised BKD chapter for OIE Diagnostic Manual for Aquatic Animal Diseases.
Revised virology portion of new diagnostic manual for aquatic animal diseases for use by US entities.
Invited member of task force for development of national aquatic animal health program for US.
Preparation and supply of international reference standards for diagnostic tests or vaccines
1. Testing and distribution of reference cell lines.
2. Testing and distribution of reference polyclonal and monoclonal antisera.
3. Testing and distribution of reference reagents for polymerase chain reaction.
5.
Research and development of new procedures for diagnosis and control
1. Development of monoclonal antibodies against IHNV.
2. Development of biotinylated DNA probes for IHNV.
3. Development of polymerase chain reaction primer sets for IHNV.
4. Comparing genomic sequences of IHNV strains from throughout the world.
5. Identification of neutralizing epitopes on the IHNV glycoprotein.
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
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Infectious haematopoietic necrosis
6. Comparative challenge studies with various isolates of IHNV.
7. Killed vaccine studies in fish for control of IHN.
8. DNA vaccine studies in fish for control of IHN via genetic immunization.
9. Development of quantitative PCR assay for IHNV.
10. Development of quantitative PCR for trout cytokine genes involved in innate resistance to IHNV.
11. Development of strand-specific qPCR assay for messenger and genomic RNA species.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
1. Provide data and professional advice to US Dept of Agriculture for OIE Annual Report.
7.
Provision of consultant expertise to OIE or to OIE Members
1. Provided advice to laboratory in Singapore
2. Provided advice to laboratory in Taiwan
3. Provided advice to laboratory in Japan
4. Provided advice to laboratory in Sri Lanka
8.
Provision of scientific and technical training to personnel from other OIE Members
1. Hosted personnel from laboratories in Canada, Norway and Philippines.
9.
Provision of diagnostic testing facilities to other OIE Members
–
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
–
11. Participation in international scientific collaborative studies
1. Participated in collaborative study with personnel from Department of Fisheries and Oceans, Canada.
2. Participated in collaborative study with personnel from National Veterinary Laboratory, Denmark.
3. Participated in collaborative study with personnel from National Veterinary Laboratory, Norway.
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Scientific publications in peer-reviewed journals
Hershberger, P.K., J. Gregg, C. Grady, R. Collins and J. Winton (in press) Viral shedding is a key driver in the
ecology of an acute infectious disease in marine fish. Prog. Mar. Ecol.
Johansson, T., K. Einer-Jensen, W. Batts, P. Ahrens, C. Bjorkblom, G. Kurath, H. Bjorklund and N. Lorenzen
(2009) Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates. Dis.
Aquat. Org. 86:213-221.
Kent, M.L., S.W. Feist, C. Harper, S. Hoogstraten-Miller, J. MacLaw, J.M. Sanchez-Morgado, R.L. Tanguay, G.E.
Sanders, J.M. Spitsbergen and C.M. Whipps (2009) Recommendations for control of pathogens and infectious
diseases in fish rearing facilities. Comp. Biochem. Physiol. Part C. 149:240-248.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
Infectious haematopoietic necrosis
Kurath, G., K.A. Garver, S.E. LaPatra and M.K. Purcell (in review) Protective immunity in Redfish Lake sockeye
salmon (Oncorhynchus nerka) challenged with U and M genogroup strains of infectious hematopoietic necrosis
virus. J. Fish Dis.
Park, J.W., C.H. Moon, A.R. Wargo, M.K. Purcell and G. Kurath (in review) Differential growth of U and M type
infectious hematopoietic necrosis virus in a rainbow trout derived cell line, RTG-2. J. Fish Dis.
Penaranda, M.D., M.K. Purcell and G. Kurath (2009) Differential virulence mechanisms of infectious
hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication
kinetics. J. Gen. Virol. 90:2172-2182.
Polinski, M.P., J.D. Drennan, W.N. Batts, S.C. Ireland and K.D. Cain (in preparation) Establishment of a cell line
from burbot Lota lota with characterization of susceptibility to IHNV, IPNV and VHSV
Purcell, M.K., K.J. Laing, J.C. Woodson, G.H. Thorgaard and J.D. Hansen (2009) Characterization of the
interferon genes in homozygous rainbow trout reveals two novel genes, alternate splicing and differential
regulation of duplicated genes. Fish Shellfish Immunol. 26:293-304.
Purcell, M.K., K.A. Garver, C. Conway, D.G. Elliott and G. Kurath (2009) Infectious hematopoietic necrosis virus
genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish
Lake Idaho. J. Fish Dis. 32:619-631.
Purcell, M. K., S. E. LaPatra, J. C. Woodson, G. Kurath and J. Winton (in review) Differential susceptibility of
rainbow trout (Oncorhynchus mykiss) families to infectious hematopoietic necrosis virus (IHNV) is associated
with early viral replication and suggests a role for constitutive defenses. Fish Shellfish Immunol.
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register?
No.
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register?
No.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
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