OIE Reference Laboratory Reports
Activities in 2010
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Koi herpesvirus disease
National Research Institute of Aquaculture,
Fisheries Research Agency
Nakatsuhamaura, Minami-Ise, Watarai, Mie 516-0193, JAPAN
Tel.:
(+81-599) 66.18.30
Fax:
(+81-599) 66.19.62
e-mail address:
KHV-lab@fra.affrc.go.jp
website:
Name of Head of Laboratory
(Responsible Official):
Dr Kei Yuasa
Name of OIE Reference Expert:
Dr Kei Yuasa
Name of writer of this report
(if different from above):
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
1
Koi herpesvirus disease
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease at your laboratory
Test
For
Specificity
Total
PCR
DNA
Type
65
TaqMan quantitative PCR
DNA
Type
446
ELISA
antibody
Group
133
KF-1 or CCB cell culture
Virus isolation
14
Production and distribution of diagnostic reagents
Reference carp cell line, virus strain, ethanol-fixed tissue of diseased fish for PCR, plasmid inserted with cDNA
for PCR positive control and ELISA system.
1.
Virus DNA was supplied to Freshwater Aquaculture Center and College of Fisheries Central Luzon State
University, Philippines; Laboratory of virology, Veterinary Research institute of Tunisia; College of Tropical
Agriculture and Human Resources, Department of Molecular Biosciences and Bioengineering, USA; the
Inspection and Quarantine Agriculture, Fisheries and Conservation Department, The Government of Hong
Kong SAR; National Fisheries Research & Development, South Korea; Mediterranean Fisheries Research,
Production and Training Institute, Ministry of Agriculture and Rural Affairs, Republic of Turkey.
2.
Plasmids inserted with cDNA for PCR positive control were supplied to 1 domestic governmental research
institutes.
3.
A cell line of carp fin was supplied to Lab of Aquatic Medicine, College of Veterinary Medicine, Seoul
National University
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
Participated a worldwide interlaboratory ring-test conducted by Dr Niels Jorgen Olesen and Nicole Nicolajsen of
EU Reference Laboratory for Fish Diseases.
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
1. Reference cell line for virus isolation: KF-1 cell line (originated to Dr R. P. Hedrick, UC Davis, USA), CCB
cell line (originated to Dr M. Neukirch, School of Veterinary Medicine Hanover, Germany), and KKE, CF-1,
NGF-2,-3, LBCB-1,2 and LBCE (originated to our laboratory).
2. Virus strain
3. Ethanol-fixed tissue of diseased fish for PCR positive control
4. Plasmid inserted with cDNA of PCR target region for PCR or TaqMan PCR positive control
5. ELISA system for detection of carp antibodies against the virus
2
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
Koi herpesvirus disease
5.
Research and development of new procedures for diagnosis and control
1. Verification of a RT-PCR assay detecting mRNA of the virus
2. Study on fate of the virus in fish and appropriate target tissues for PCR detection of subclinical carrier fish
3. Improvement of ELISA system for detection of carp antibodies against the viral antigens
4. Epizootiological study on the disease among fish population in natural waters
5. Development of more susceptible cell line for the virus isolation and quantification
6. Comparison of pathogenicity to carp among year-on-year isolates.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
None
7.
Provision of consultant expertise to OIE or to OIE Members
None
8.
Provision of scientific and technical training to personnel from other OIE Members
Transferring cell culture techniques for propagation of KHV on carp cell line and PCR diagnostic techniques to
one researcher of Mediterranean Fisheries Research, Production and Training Institute, Ministry of Agriculture
and Rural Affairs, Republic of Turkey; to one researcher of the Inspection and Quarantine Agriculture, Fisheries
and Conservation Department, The Government of Hong Kong SAR; and to two researcher of National Fisheries
Research & Development, South Korea.
9.
Provision of diagnostic testing facilities to other OIE Members
None
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
None
11. Participation in international scientific collaborative studies
None
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)
None
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
3
Koi herpesvirus disease
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register? If yes, for which kits?
No
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
which kits?
No
_______________
4
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008