Table S2. Primers used for constructing and confirming the mutants and for protein expression
Primer
Gene
Sequence(5‘-3‘)
For screening library
ORF5317-For
ORF5317-Rev
ORF5310-For
ORF5310-Rev
ORF5317
ORF5310
GCGTGCGGACATGCGTGTTGTGGTG
GGGTCCAACCTACCCAAATGTGATGGAGCAA
GCGGCTGGAGTGTAGCGAGTCTGGAATG
GGGTATGTCCACCCGATGCTCCTGTCTCAT
For PCR Targeting
TORF5311-For
TORF5311-Rev
TORF5313-For
TORF5313-Rev
TORF5314-For
TORF5314-Rev
TORF5315-For
TORF5315-Rev
TORF5316-For
TORF5316-Rev
ximA
ximB
ximC
ximD
ximE
ATGAGACAGGAGCATCGGGTGGACATACCCGAGAACTTGTGGTTCATGTGCAGCTCCATC
TCACGTTCGAGGCGCATTCGACGCCGGATAGTGACGATG TGAGCTCAGCCAATCGACTG
GTGATCGATATTTCCGCTCAACCCTCGCAGCAGAGCACGTGGTTCATGTGCAGCTCCATC
TCAAAAGACTCTCCCCGCAACGATGGCGACGAGCACGAGTGAGCTCAGCCAATCGACTG
GTGCGCACGGAGTCGCGCAGCCTGGCCCAGTTCGTGGCGTGGTTCATGTGCAGCTCCATC
TCATGCGTCGTGGACGGCGTCTCGATCGAGGAGACACGGTGAGCTCAGCCAATCGACTG
ATGCCGAACTCTCCCGCCGCGGTCTTCGAGCGGCTCACCTGGTTCATGTGCAGCTCCATC
TCACGTCGTCTCCATCATCGTGTACTCCTGCCGGATCCGTGAGCTCAGCCAATCGACTG
ATGGGCCAGACGACGCACACAGCACTCGACCGCTACATGTGGTTCATGTGCAGCTCCATC
TCAGCCCGGCGTACGGGTGTACCGGTTGCGCAGGTTCGTTGAGCTCAGCCAATCGACTG
For gene verification
VORF5311-For
VORF5311-Rev
VORF5313-For
VORF5313-Rev
VORF5314-For
VORF5314-Rev
VORF5315-For
VORF5315-Rev
VORF5316-For
ximA
ximB
ximC
ximD
ximE
GCCCGAACCCCTGAGACTCTGGAGATC
GCCGCCTTGTCCTAACGGTGTTTGCC
CCGAGGTGGGCTTTCTGCAAGAGTCA
GCACGGACCAGGGGCCTGCAT
CTGCCGGGAAAAGTTCATGGCAAACCG
TGCCGAGACGAACCTCGATGCGGAC
GCCGTCCACGACGCATGAGGGATC
GGTCGGCGAGCTCCATGTAGCGGTC
CGGCAGGAGTACACGATGATGGAGACG
1
VORF5316-Rev
TCTCGGCAGCTACCGGCGCATC
For heterologous expression
HORF5311-6-For
HORF5311-6-Rev
ximA~ximE
GCTCTAGACGGCTGGAGTGTAGCGAGTCTGGAATG
GGAATTCCCCGGACGTGGGAGCGATAGGG
For protein expression
ximA-F-NdeⅠ
ximA
ximA-R-XhoⅠ
ximB-F-NdeⅠ
ximB
ximB-R-XhoⅠ
ximC-F-NdeⅠ
ximC
ximC-R-HindⅢ
ximD-F-EcoRⅠ
ximD
ximD-R-HindⅢ
ximE-F-NdeⅠ
ximE
ximE-R- HindⅢ
TCAGCcatATGAGACAGGAGCATCGGGTGG
TCAGctcgagTCACGTTCGAGGCGCATTC
ctagcataTGGTGATCGATATTTCCGCTC
atcgctcgagTCAAAAGACTCTCCCCGCAAC
ataccataTGCGCACGGAGTCG
atacaagcttTCATGCGTCGTGGACG
TCAGgaattcATGCCGAACTCTCCCGC
TCAGaagcttTCACGTCGTCTCCATCATCG
TCGAcatATGGGCCAGACGACGCACAC
TCAGaagcttTCAGCCCGGCGTACGGGT
For protein coexpression
ximD-F-EcoRⅠ
ximD
ximD-R-HindⅢ
CoximE-F-NdeⅠ
ximE
CoximE-R-KpnⅠ
TCAGgaattcATGCCGAACTCTCCCGC
TCAGaagcttTCACGTCGTCTCCATCATCG
TCGAcatATGGGCCAGACGACGCACAC
TAGCggtaccTCAGCCCGGCGTACGGGT
For RT-PCR
ORF1467
ORF1467
ORF2290
ORF2290
ORF2781
ORF2781
GCCAACGCCCCCTCACCTC
GCCGACCACCATCATGCTC
GCGTCGGCGAAGTCGTTGA
GCGTCCGTCTGCCTCTACC
ACCTCGGCACCCGCACCAC
2
CGGCCACCAGTCCCACTCC
ORF4925
ORF4925
ACCGTCACCCCGCACAAGT
GCCATCCAGCAGAAGAACA
ORF5065
ORF5065
ORF5313
ORF5313
ACGTGGTGATGTTCGAGCA
GTCAGGGAGGCGGTGTAGG
TGGCTGGGGATGGTCTTCG
CCTTGTCCTGGTGGGCGTA
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