Table S3. Primers used in this study
Primer
F/R
369
354
373
372
350
353
376
352
391
392
410
411
408
409
404
407
420
421
416
417
64
206
207
MmPitx2cF
MmPitx2cR
MmFoxj1F
MmFoxj1R
MmMyf5F
MmMyf5R
MmPax3F
MmPax3R
MmMyogF
MmMyogR
MmGapdhF
MmGapdhR
MmGusbF
MmGusbR
F
R
F
F
F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
R
F
R
F
R
F
R
F
R
F
R
F
R
F
R
Target
Pitx2c
Pitx2
Pitx2b
Pitx2a
Pitx2
Pitx2
Pitx2c
Pitx2
Pitx2c
Pitx2c
Myf5
Myf5
Myf5
Myf5
Myog
Myog
Foxj1
Foxj1
Pax3
Pax3
Rl19
Rpl19
Rpl19
pitx2c
pitx2c
Foxj1
Foxj1
Myf5
Myf5
Pax3
Pax3
Myog
Myog
Gapdh
Gapdh
Gusb
Gusb
Species
Sequence (5´-3´)
P
P
P
P
P
P
P
P
H
H
P
P
H
H
P/H
P/H
P
P
P
P
P/H
P
H
M
M
M
M
M
M
M
M
M
M
M
M
M
M
tgaaaggcccgctgcacttg
gaggagttggcggtgcgtaag
gcagccagcagcaagctcttc
cggcgtgtgtgcaattagagaaagac
catgtccacgcgtgaagaaatc
tgcggcccaaagccattcttg
gcacaccatctctgacacctccag
tttggcccggcgattcttgaac
gcacaccatctccgacacctccag
tgccgcttcttcttagacgggtcctc
cagcagttttgacagtatctactgtcc
atccgatccactatgctggataagc
gcagtacttttgacagcatctactgtcct
actatgttggataagcaatccaagctgg
cacagcgcctcctgcagtc
catggtttcatctgggaaggccacag
cagcaaggccaccaagatcac
agcacttgttcagagacaggttgtg
accttcacagcagagcagcttg
atggttgaaagccatcagttgattgg
ctgctcagaagataccgtgaat
gcttgtggatgtgctccatga
gcttgtggatgtgttccatga
cctcacccttctgtcaccat
gcccacatcctcattctttc
acacgtgaagccaccctact
tgttcaaggacaggttgtgg
tgagggaacaggtggagaac
agctggacacggagctttta
gccaatcaactgatggcttt
gtacagtgctcggaggaagc
ctacaggccttgctcagctc
acgatggacgtaagggagtg
ggcattgctctcaatgacaa
ggcattgctctcaatgacaa
acgcatcagaagccgattat
actctcagcggtgactggtt
Product length
Application
369-354: 825 bp (Pitx2c)
373-354: 670 bp (Pitx2b)
372-354: 642 bp (Pitx2a)
RT-PCR
RT-PCR
RT-PCR
350-353: 144 bp (Pitx2)
qPCR
376-352: 271 bp (Pitx2c)
qPCR
391-392: 108 bp
qPCR
410-411: 105 bp
qPCR
408-409: 97 bp
qPCR
404-407: 174 bp
qPCR
420-421:128 bp
qPCR
416-417:192 bp
qPCR
64-206:121 bp
64-207:121 bp
qPCR
qPCR
179 bp
qPCR
176 bp
qPCR
198 bp
qPCR
181 bp
qPCR
221 bp
qPCR
200 bp
qPCR
212 bp
qPCR
F/R – forward/reverse primers; P – pig; H – human: M – mouse.